Detailed information of TA system TA063061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 49671..50295
• Replicon: plasmid unnamed1
• Accession: NZ_CP015419
• Organism: Rhodovulum sulfidophilum DSM 1374

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A6W98_RS19350
• Protein ID: WP_042465586.1
• Coordinates: 49891..50295 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A6W98_RS19345
• Protein ID: WP_042465583.1
• Coordinates: 49671..49901 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6W98_RS19325	45771..46277	-	507	WP_042465573.1	TRAP transporter small permease	-
A6W98_RS19330	46378..47535	-	1158	WP_042465576.1	TRAP transporter substrate-binding protein	-
A6W98_RS19335	47579..47803	-	225	WP_042465579.1	hypothetical protein	-
A6W98_RS19340	47917..48960	+	1044	WP_042465581.1	LacI family DNA-binding transcriptional regulator	-
A6W98_RS19345	49671..49901	+	231	WP_042465583.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A6W98_RS19350	49891..50295	+	405	WP_042465586.1	PIN domain-containing protein	Toxin
A6W98_RS19355	50404..51075	+	672	WP_042465589.1	SDR family NAD(P)-dependent oxidoreductase	-
A6W98_RS19360	51089..51934	-	846	WP_155734912.1	DMT family transporter	-
A6W98_RS19365	52267..52887	+	621	WP_042465595.1	exopolysaccharide biosynthesis protein	-
A6W98_RS19370	53371..53994	+	624	WP_042465597.1	RNA ligase family protein	-
A6W98_RS19375	54507..55148	-	642	WP_042465600.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113163	113163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14622.72 Da
• Isoelectric Point: 4.3913

>T63061 WP_042465586.1 NZ_CP015419:49891-50295 [Rhodovulum sulfidophilum DSM 1374]
MPADFFDTNVILYLLDDGPKADRSEALLAKGGTISVQVLNEALVNCLRKARMSHDEAGEFLFGIRDLCETVELTVDTHDV
GRALAARYKLSVYDSMIVAAALLSGCTRLWSEDMQDGLTVEGRLKIVNPFADAA

Nucleotide

• Length: 405 bp

>T63061 NZ_CP015419:49891-50295 [Rhodovulum sulfidophilum DSM 1374]
ATGCCCGCTGACTTCTTCGACACCAACGTCATCCTCTACCTGCTGGACGACGGGCCCAAGGCCGACCGGTCCGAGGCGCT
GCTGGCTAAGGGCGGCACGATCAGCGTCCAGGTGCTGAACGAGGCGCTGGTGAACTGCCTGCGCAAGGCCCGGATGAGCC
ATGACGAGGCGGGCGAATTCCTGTTCGGGATCCGCGATCTCTGCGAGACGGTCGAGCTGACCGTCGACACCCATGATGTC
GGCCGCGCGCTGGCCGCCCGCTACAAGCTGTCGGTCTACGATTCGATGATCGTCGCCGCGGCGCTTCTCAGCGGCTGCAC
CAGGCTCTGGTCCGAGGACATGCAAGACGGGCTGACCGTCGAGGGGCGGCTGAAGATCGTCAACCCCTTCGCCGACGCCG
CCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8490.63 Da
• Isoelectric Point: 11.8979

>AT63061 WP_042465583.1 NZ_CP015419:49671-49901 [Rhodovulum sulfidophilum DSM 1374]
MQIARWGNSLAVRLPADLVRTLGLREGDRIELEPSGEGRLAVSRHPRADEVLSGLRRFRGRLASEDRLSRDAANAR

Nucleotide

• Length: 231 bp

>AT63061 NZ_CP015419:49671-49901 [Rhodovulum sulfidophilum DSM 1374]
ATGCAGATCGCCAGATGGGGAAATTCGCTTGCCGTCCGCCTGCCCGCGGATCTGGTCCGGACCCTCGGCCTGCGCGAGGG
CGACCGGATCGAGCTGGAGCCCTCGGGCGAGGGTCGGCTTGCAGTCAGCCGCCATCCCCGTGCCGATGAGGTGCTGTCAG
GGCTGCGCCGGTTCCGCGGCCGTCTGGCGTCCGAAGACCGGCTGAGCCGCGACGCGGCCAATGCCCGCTGA