Detailed information of TA system TA063036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 18787..19430
• Replicon: plasmid pCR14_3
• Accession: NZ_CP015395
• Organism: Klebsiella pneumoniae strain CR14

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: A6P37_RS29630
• Protein ID: WP_001044770.1
• Coordinates: 19014..19430 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: A6P37_RS29625
• Protein ID: WP_001261282.1
• Coordinates: 18787..19017 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P37_RS29580	13985..14458	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
A6P37_RS29585	14550..14780	+	231	WP_011977773.1	hypothetical protein	-
A6P37_RS29590	15672..16454	-	783	WP_004152340.1	site-specific integrase	-
A6P37_RS29595	16454..16786	-	333	WP_004152339.1	hypothetical protein	-
A6P37_RS29600	16793..17191	-	399	WP_004171440.1	hypothetical protein	-
A6P37_RS29605	17217..17546	-	330	WP_004152337.1	hypothetical protein	-
A6P37_RS29610	17574..17882	-	309	WP_004152336.1	hypothetical protein	-
A6P37_RS29620	18369..18830	-	462	WP_072093212.1	hypothetical protein	-
A6P37_RS29625	18787..19017	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A6P37_RS29630	19014..19430	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
A6P37_RS29635	19504..20214	+	711	WP_004152334.1	ATP-binding protein	-
A6P37_RS29640	20248..20945	+	698	Protein_22	IS1 family transposase	-
A6P37_RS29645	20961..21071	+	111	Protein_23	mercuric transport protein periplasmic component	-
A6P37_RS29650	21107..21529	+	423	WP_001340589.1	organomercurial transporter MerC	-
A6P37_RS29655	21581..23275	+	1695	WP_000105636.1	mercury(II) reductase	-
A6P37_RS29660	23293..23655	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
A6P37_RS29665	23652..23888	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A / blaOXA-9 / blaKPC-2	-	1..116419	116419

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T63036 WP_001044770.1 NZ_CP015395:19014-19430 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T63036 NZ_CP015395:19014-19430 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT63036 WP_001261282.1 NZ_CP015395:18787-19017 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT63036 NZ_CP015395:18787-19017 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3