Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 20247..20983 Replicon plasmid pCR14_1
Accession NZ_CP015393
Organism Klebsiella pneumoniae strain CR14

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag A6P37_RS27510 Protein ID WP_003026803.1
Coordinates 20501..20983 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6P37_RS27505 Protein ID WP_003026799.1
Coordinates 20247..20513 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6P37_RS27460 16309..16671 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
A6P37_RS27465 16721..17071 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
A6P37_RS27470 17429..17698 + 270 WP_004152102.1 hypothetical protein -
A6P37_RS27475 17686..18261 + 576 WP_004152103.1 hypothetical protein -
A6P37_RS27480 18292..18786 + 495 WP_004152104.1 DNA-binding protein -
A6P37_RS27485 18830..19198 + 369 WP_004152105.1 hypothetical protein -
A6P37_RS27490 19232..19435 + 204 WP_004152106.1 hemolysin expression modulator Hha -
A6P37_RS27495 19484..19741 + 258 WP_004152107.1 hypothetical protein -
A6P37_RS27500 19817..20071 + 255 WP_004152108.1 hypothetical protein -
A6P37_RS27505 20247..20513 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6P37_RS27510 20501..20983 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
A6P37_RS27515 21195..22541 + 1347 WP_020314316.1 ISNCY family transposase -
A6P37_RS31440 23042..24296 - 1255 Protein_31 IS3 family transposase -
A6P37_RS27530 24384..25346 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / mph(A) - 1..202696 202696


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T63034 WP_003026803.1 NZ_CP015393:20501-20983 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T63034 NZ_CP015393:20501-20983 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT63034 WP_003026799.1 NZ_CP015393:20247-20513 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT63034 NZ_CP015393:20247-20513 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References