Detailed information of TA system TA063015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 28454..29016
• Replicon: plasmid pNY9_5
• Accession: NZ_CP015390
• Organism: Klebsiella pneumoniae strain NY9

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: A6P56_RS31515
• Protein ID: WP_045325297.1
• Coordinates: 28699..29016 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: A6P56_RS31510
• Protein ID: WP_001623606.1
• Coordinates: 28454..28699 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P56_RS31470	24238..24567	+	330	WP_045325287.1	hypothetical protein	-
A6P56_RS31480	24970..25494	+	525	WP_001610342.1	DnaJ domain-containing protein	-
A6P56_RS31485	25544..26107	+	564	WP_045325289.1	hypothetical protein	-
A6P56_RS31490	26175..26471	+	297	WP_045325291.1	hypothetical protein	-
A6P56_RS31495	26455..26958	+	504	WP_045325293.1	hypothetical protein	-
A6P56_RS34215	27600..27827	-	228	WP_157843658.1	hypothetical protein	-
A6P56_RS34455	27971..28144	+	174	WP_001610337.1	hypothetical protein	-
A6P56_RS31510	28454..28699	+	246	WP_001623606.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A6P56_RS31515	28699..29016	+	318	WP_045325297.1	CcdB family protein	Toxin
A6P56_RS31520	29120..29368	+	249	WP_045325298.1	hypothetical protein	-
A6P56_RS31525	29372..29584	+	213	Protein_35	hypothetical protein	-
A6P56_RS31530	29601..29906	+	306	WP_045325303.1	hypothetical protein	-
A6P56_RS31535	30098..30556	-	459	WP_045325304.1	hypothetical protein	-
A6P56_RS31545	31121..31885	+	765	WP_037404221.1	tyrosine-type recombinase/integrase	-
A6P56_RS31550	31999..32664	+	666	WP_001623616.1	hypothetical protein	-
A6P56_RS31555	32710..33030	+	321	WP_045325308.1	hypothetical protein	-
A6P56_RS31560	33422..33901	-	480	WP_001773442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39432	39432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (9-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11798.77 Da
• Isoelectric Point: 8.4499

>T63015 WP_045325297.1 NZ_CP015390:28699-29016 [Klebsiella pneumoniae]
VKQFAVYKNKSRNKQAYPYFIDVQADMLAHLNTRLVMPLTQKSNSNSQVKALTPIITIDQIDYVVLATMITTTDVKNLKA
EDAVMDASHLRDQLVSAIDMMILGI

Nucleotide

• Length: 318 bp

>T63015 NZ_CP015390:28699-29016 [Klebsiella pneumoniae]
GTGAAACAGTTTGCAGTTTACAAAAATAAATCCCGGAACAAACAGGCATACCCGTATTTTATTGATGTGCAGGCAGATAT
GCTTGCACACCTTAATACTCGCCTAGTTATGCCTTTAACGCAGAAATCTAACTCTAATTCGCAGGTAAAAGCGTTAACGC
CAATTATTACAATCGATCAGATCGATTATGTAGTGCTGGCAACCATGATAACGACAACGGATGTAAAAAATCTGAAAGCT
GAAGATGCAGTTATGGATGCTTCACATTTACGCGATCAATTGGTTTCTGCGATTGATATGATGATTTTAGGTATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9198.25 Da
• Isoelectric Point: 4.4243

>AT63015 WP_001623606.1 NZ_CP015390:28454-28699 [Klebsiella pneumoniae]
MRTNVAEKKRSTNVYLNAGLLEEAKELDLNVSAISNQALETAVRERKRERWIEENRAGIEALNSFVEEVGIFSDDENFGV
I

Nucleotide

• Length: 246 bp

>AT63015 NZ_CP015390:28454-28699 [Klebsiella pneumoniae]
ATGAGAACGAATGTAGCCGAGAAAAAACGTAGTACAAACGTGTACTTAAATGCTGGCCTACTTGAAGAGGCGAAAGAGCT
GGACTTGAACGTTTCCGCGATATCTAACCAGGCGCTGGAAACGGCTGTCAGAGAACGCAAGCGTGAACGTTGGATCGAGG
AAAACCGCGCGGGCATCGAGGCGTTAAACAGCTTCGTGGAAGAGGTGGGTATTTTTTCAGATGATGAAAATTTCGGGGTG
ATTTAG