Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1487868..1488604 Replicon chromosome
Accession NZ_CP015382
Organism Klebsiella pneumoniae strain CN1

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag A6U99_RS07715 Protein ID WP_032433360.1
Coordinates 1488122..1488604 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6U99_RS07710 Protein ID WP_003026799.1
Coordinates 1487868..1488134 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6U99_RS07685 1483514..1484653 + 1140 WP_032433368.1 mannitol dehydrogenase -
A6U99_RS07690 1484682..1485344 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
A6U99_RS07695 1485328..1486332 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
A6U99_RS07700 1486350..1486982 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
A6U99_RS07705 1486992..1487555 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
A6U99_RS07710 1487868..1488134 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6U99_RS07715 1488122..1488604 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
A6U99_RS07720 1488965..1489564 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
A6U99_RS07725 1489777..1490721 - 945 WP_077254249.1 fimbrial protein -
A6U99_RS07730 1490733..1491311 - 579 WP_032432061.1 type 1 fimbrial protein -
A6U99_RS07735 1491315..1492055 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1453681..1499547 45866


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T62976 WP_032433360.1 NZ_CP015382:1488122-1488604 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T62976 NZ_CP015382:1488122-1488604 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT62976 WP_003026799.1 NZ_CP015382:1487868-1488134 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT62976 NZ_CP015382:1487868-1488134 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References