Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1470111..1470754 Replicon chromosome
Accession NZ_CP015382
Organism Klebsiella pneumoniae strain CN1

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag A6U99_RS07610 Protein ID WP_032433387.1
Coordinates 1470338..1470754 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag A6U99_RS07605 Protein ID WP_001261276.1
Coordinates 1470111..1470341 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6U99_RS07580 1465133..1466220 + 1088 Protein_1409 transcriptional regulator -
A6U99_RS07585 1466223..1468463 + 2241 WP_045326788.1 NTPase -
A6U99_RS07595 1468991..1469806 - 816 WP_032433388.1 hypothetical protein -
A6U99_RS07605 1470111..1470341 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A6U99_RS07610 1470338..1470754 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
A6U99_RS07615 1470910..1471890 + 981 WP_032433385.1 hypothetical protein -
A6U99_RS07620 1472085..1473656 - 1572 WP_032433383.1 ATP-binding protein -
A6U99_RS07625 1473975..1474223 + 249 WP_032433382.1 hypothetical protein -
A6U99_RS07630 1474282..1474800 + 519 WP_045326794.1 hypothetical protein -
A6U99_RS07635 1474831..1475322 + 492 WP_032433378.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1453681..1499547 45866
- flank IS/Tn - - 1475418..1476341 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T62975 WP_032433387.1 NZ_CP015382:1470338-1470754 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T62975 NZ_CP015382:1470338-1470754 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT62975 WP_001261276.1 NZ_CP015382:1470111-1470341 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT62975 NZ_CP015382:1470111-1470341 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References