Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/- |
| Location | 2379061..2380288 | Replicon | chromosome |
| Accession | NC_004722 | ||
| Organism | Bacillus cereus ATCC 14579 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | W8Y388 |
| Locus tag | BC_RS12185 | Protein ID | WP_000624977.1 |
| Coordinates | 2379061..2379798 (+) | Length | 246 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | () | Length | 0.33333333333333 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BC_RS12160 (BC2431) | 2374674..2375081 | + | 408 | WP_000063587.1 | VOC family protein | - |
| BC_RS12165 (BC2432) | 2375105..2375485 | + | 381 | WP_000497668.1 | methyltransferase | - |
| BC_RS12170 (BC2433) | 2375643..2377328 | - | 1686 | WP_002195513.1 | alpha-keto acid decarboxylase family protein | - |
| BC_RS12175 (BC2434) | 2377436..2377918 | + | 483 | WP_000191888.1 | MarR family winged helix-turn-helix transcriptional regulator | - |
| BC_RS12180 (BC2435) | 2378085..2378822 | + | 738 | WP_000594152.1 | 2,3-diphosphoglycerate-dependent phosphoglycerate mutase | - |
| BC_RS12185 (BC2436) | 2379061..2379798 | + | 738 | WP_000624977.1 | stage II sporulation protein SA | Toxin |
| BC_RS12190 | 2379911..2380039 | + | 129 | WP_000237819.1 | hypothetical protein | - |
| BC_RS12195 (BC2437) | 2380112..2380288 | + | 177 | WP_000852629.1 | stage II sporulation protein SB | - |
| BC_RS12200 (BC2438) | 2380308..2380697 | - | 390 | WP_000713863.1 | YxeA family protein | - |
| BC_RS12205 (BC2439) | 2380909..2382378 | + | 1470 | WP_000287556.1 | beta-Ala-His dipeptidase | - |
| BC_RS12210 (BC2440) | 2382624..2383433 | + | 810 | WP_000664546.1 | C39 family peptidase | - |
| BC_RS12215 (BC2441) | 2383459..2384061 | + | 603 | WP_000517266.1 | hypothetical protein | - |
| BC_RS12220 (BC2442) | 2384305..2384556 | - | 252 | WP_000827560.1 | response regulator transcription factor | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 246 a.a. Molecular weight: 28307.03 Da Isoelectric Point: 8.2998
>T6297 WP_000624977.1 NC_004722:2379061-2379798 [Bacillus cereus ATCC 14579]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK
Download Length: 738 bp
>T6297 NC_004722:2379061-2379798 [Bacillus cereus ATCC 14579]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTTTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATCGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTTTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATCGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA
Antitoxin
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Patrik Florek et al. (2008) Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli. FEMS Microbiology Letters 278(2):177-84. [PubMed:18096016]
(2) Jana Melničáková et al. (2015) Analysis of the Bacillus cereus SpoIIS antitoxin-toxin system reveals its three-component nature. Frontiers in Microbiology 6:808. [PubMed:26300872]