Detailed information of TA system TA062964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 4234369..4235126
• Replicon: chromosome
• Accession: NZ_CP015379
• Organism: Hafnia alvei strain HUMV-5920

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: A6V27_RS19240
• Protein ID: WP_040046925.1
• Coordinates: 4234644..4235126 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A2J9H2I2
• Locus tag: A6V27_RS19235
• Protein ID: WP_040046924.1
• Coordinates: 4234369..4234653 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6V27_RS19210	4229692..4230528	+	837	WP_040046955.1	ABC transporter substrate-binding protein	-
A6V27_RS19215	4230574..4231071	+	498	WP_040046920.1	heme utilization cystosolic carrier protein HutX	-
A6V27_RS19220	4231189..4232163	+	975	WP_145916012.1	iron ABC transporter permease	-
A6V27_RS19225	4232211..4232783	-	573	WP_040046922.1	DedA family protein	-
A6V27_RS19230	4233075..4234184	+	1110	WP_040046923.1	sodium-potassium/proton antiporter ChaA	-
A6V27_RS19235	4234369..4234653	+	285	WP_040046924.1	DUF1778 domain-containing protein	Antitoxin
A6V27_RS19240	4234644..4235126	+	483	WP_040046925.1	GNAT family N-acetyltransferase	Toxin
A6V27_RS19245	4235147..4235989	-	843	WP_063587348.1	DMT family transporter	-
A6V27_RS19250	4236142..4236930	-	789	WP_025801705.1	phosphate starvation-inducible protein PhoH	-
A6V27_RS19255	4237454..4237888	+	435	WP_025801704.1	Rrf2 family transcriptional regulator	-
A6V27_RS19260	4237978..4238622	-	645	WP_025801703.1	protein phosphatase CheZ	-
A6V27_RS19265	4238632..4239021	-	390	WP_025801702.1	chemotaxis response regulator CheY	-
A6V27_RS19270	4239057..4240109	-	1053	WP_025801701.1	chemotaxis response regulator protein-glutamate methylesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	chuX / chuU / cheZ / cheY / cheB / cheR / cheD / cheW / cheA / motB / motA / flhC / flhD	4145893..4286018	140125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-93)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17919.58 Da
• Isoelectric Point: 7.8716

>T62964 WP_040046925.1 NZ_CP015379:4234644-4235126 [Hafnia alvei]
MGINITSPELLTEEHELQSFNSGNDVLNDWLRRRAIKNQYLNASRTFVICLECTNRVVGYYSLATGSVSHTDLGRGLRQN
MPNPIPVILLGRLAVDVSTQGNNFGKWLLKDAVMRVSNLADLVGIKAIMVHAINEQAQAFYEYFGFVQSPIAPNTLFYKI

Nucleotide

• Length: 483 bp

>T62964 NZ_CP015379:4234644-4235126 [Hafnia alvei]
ATGGGAATAAATATCACGTCTCCTGAGCTACTGACCGAAGAGCACGAACTACAATCTTTCAACAGTGGAAATGATGTACT
CAATGATTGGCTTCGCCGCCGCGCCATAAAAAATCAGTACCTTAATGCCTCCCGCACCTTTGTTATTTGCCTAGAATGTA
CCAATCGCGTCGTTGGCTATTACTCCCTTGCGACCGGCTCAGTAAGCCACACCGATTTGGGACGTGGCTTGCGCCAGAAT
ATGCCAAACCCCATACCTGTTATTCTGCTGGGCCGCCTTGCCGTTGACGTATCTACTCAGGGTAATAATTTTGGGAAATG
GTTACTTAAGGATGCGGTGATGCGCGTCTCTAATCTGGCCGATCTGGTGGGAATTAAGGCCATTATGGTACATGCCATCA
ATGAGCAAGCGCAGGCGTTCTATGAATACTTTGGTTTTGTTCAGTCTCCCATTGCGCCAAACACGCTGTTCTACAAAATT
TAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11027.45 Da
• Isoelectric Point: 5.1974

>AT62964 WP_040046924.1 NZ_CP015379:4234369-4234653 [Hafnia alvei]
MQTTPRKSTRNKQINVRASEEERAVIDYAASLVNKNRTDFIIEKAVHEAQNIILDQRVFVLDDARYQAFIEQLEAPVQNI
EGRQRLMDVKPEWE

Nucleotide

• Length: 285 bp

>AT62964 NZ_CP015379:4234369-4234653 [Hafnia alvei]
ATGCAAACAACTCCCCGTAAATCTACTCGTAATAAGCAAATAAACGTTCGCGCCAGCGAAGAAGAGAGAGCTGTTATAGA
CTACGCTGCAAGCCTAGTGAATAAAAATCGGACGGATTTCATCATTGAGAAAGCCGTACATGAAGCCCAAAATATCATTC
TCGATCAACGTGTATTTGTATTGGACGATGCCCGTTATCAGGCTTTCATTGAACAGCTAGAAGCACCGGTTCAAAATATT
GAAGGTCGCCAACGCTTGATGGATGTGAAACCTGAATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J9H2I2