Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2753639..2754230 Replicon chromosome
Accession NZ_CP015379
Organism Hafnia alvei strain HUMV-5920

Toxin (Protein)


Gene name Hha Uniprot ID G9Y5B5
Locus tag A6V27_RS12360 Protein ID WP_004091739.1
Coordinates 2753639..2753842 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A097R537
Locus tag A6V27_RS12365 Protein ID WP_025799952.1
Coordinates 2753862..2754230 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6V27_RS12325 2748735..2749073 + 339 WP_004091732.1 P-II family nitrogen regulator -
A6V27_RS12330 2749104..2750396 + 1293 WP_181875401.1 ammonium transporter AmtB -
A6V27_RS12335 2750477..2751337 - 861 WP_063586628.1 acyl-CoA thioesterase II -
A6V27_RS12340 2751630..2752148 + 519 WP_025799960.1 YbaY family lipoprotein -
A6V27_RS12345 2752196..2752534 - 339 WP_063586629.1 MGMT family protein -
A6V27_RS12355 2753020..2753439 + 420 WP_063587596.1 DUF1311 domain-containing protein -
A6V27_RS12360 2753639..2753842 - 204 WP_004091739.1 hemolysin expression modulator Hha Toxin
A6V27_RS12365 2753862..2754230 - 369 WP_025799952.1 Hha toxicity modulator TomB Antitoxin
A6V27_RS21115 2754743..2754883 - 141 WP_004091741.1 type B 50S ribosomal protein L36 -
A6V27_RS12370 2754883..2755155 - 273 WP_025799950.1 type B 50S ribosomal protein L31 -
A6V27_RS12375 2755322..2758081 - 2760 WP_063586630.1 copper-exporting P-type ATPase CopA -
A6V27_RS12380 2758204..2758611 + 408 WP_040044373.1 Cu(I)-responsive transcriptional regulator -
A6V27_RS12385 2758656..2759108 - 453 WP_025799896.1 NfeD family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8119.38 Da        Isoelectric Point: 5.8346

>T62954 WP_004091739.1 NZ_CP015379:c2753842-2753639 [Hafnia alvei]
MTKIDYLMRLRKCTTLDTLERVIEKNKYELSDDELETFYSAADHRLAELTMNKLYDKIPAEVWKYVR

Download         Length: 204 bp

>T62954 NZ_CP015379:c2753842-2753639 [Hafnia alvei]
ATGACTAAAATTGATTACCTTATGCGCTTACGCAAGTGTACAACGTTAGACACCTTAGAGCGCGTAATTGAAAAAAACAA
ATACGAGCTCTCCGACGACGAGCTAGAAACTTTCTACTCAGCCGCCGACCATCGTTTGGCCGAACTCACCATGAACAAAC
TGTATGATAAGATCCCTGCTGAAGTTTGGAAGTATGTACGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14201.03 Da        Isoelectric Point: 4.7386

>AT62954 WP_025799952.1 NZ_CP015379:c2754230-2753862 [Hafnia alvei]
MDEYTSYQHDITELKYLCNMLYNQGMEVLSDSHHGWVNDPTAVANLQLNELNEHIATFGLTFKIKYPKSSELTEILDEYL
DETYSLFSNYSINEPELKKWLKVKGRILRYLAGEKPASALNF

Download         Length: 369 bp

>AT62954 NZ_CP015379:c2754230-2753862 [Hafnia alvei]
ATGGATGAGTACACGTCTTATCAGCACGATATAACTGAGCTGAAATACCTTTGTAATATGCTGTATAATCAGGGAATGGA
AGTTCTGAGTGATAGCCATCATGGCTGGGTTAATGACCCAACTGCTGTAGCTAATCTGCAACTCAATGAATTAAACGAAC
ATATCGCAACGTTCGGTTTAACGTTCAAGATTAAGTATCCAAAGAGTTCAGAATTAACTGAAATATTAGATGAGTATCTT
GACGAAACTTACAGTCTGTTTAGTAATTACAGCATAAATGAACCAGAACTAAAAAAATGGCTTAAAGTAAAAGGCCGTAT
TTTGCGTTATTTAGCGGGTGAAAAACCGGCATCCGCGTTGAACTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G9Y5B5

Antitoxin

Source ID Structure
AlphaFold DB A0A097R537

References