Detailed information of TA system TA062944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 7004629..7005215
• Replicon: chromosome
• Accession: NZ_CP015377
• Organism: Pseudomonas aeruginosa strain BAMCPA07-48

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: A6R75_RS32735
• Protein ID: WP_003120987.1
• Coordinates: 7004916..7005215 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A6R75_RS32730
• Protein ID: WP_003448662.1
• Coordinates: 7004629..7004919 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6R75_RS32715	7000211..7002100	+	1890	WP_063456203.1	hypothetical protein	-
A6R75_RS32720	7002097..7004073	+	1977	WP_023980483.1	DEAD/DEAH box helicase	-
A6R75_RS32725	7004214..7004558	+	345	WP_003448665.1	hypothetical protein	-
A6R75_RS32730	7004629..7004919	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
A6R75_RS32735	7004916..7005215	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6R75_RS32740	7005417..7006541	+	1125	WP_023980489.1	TcpQ domain-containing protein	-
A6R75_RS32745	7006541..7008250	+	1710	WP_063456060.1	PilN family type IVB pilus formation outer membrane protein	-
A6R75_RS32750	7008254..7009579	+	1326	WP_063456204.1	type 4b pilus protein PilO2	-
A6R75_RS32755	7009569..7010102	+	534	WP_023980466.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T62944 WP_003120987.1 NZ_CP015377:c7005215-7004916 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T62944 NZ_CP015377:c7005215-7004916 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT62944 WP_003448662.1 NZ_CP015377:c7004919-7004629 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT62944 NZ_CP015377:c7004919-7004629 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAGTCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTAA

Structures

Toxin

• AlphaFold DB: G8CP73