Detailed information of TA system TA062941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4601378..4602420
• Replicon: chromosome
• Accession: NZ_CP015377
• Organism: Pseudomonas aeruginosa strain BAMCPA07-48

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: A6R75_RS21685
• Protein ID: WP_003109777.1
• Coordinates: 4601845..4602420 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: A6R75_RS21680
• Protein ID: WP_003050245.1
• Coordinates: 4601378..4601848 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6R75_RS21645	4596770..4598188	-	1419	WP_031691871.1	TIGR03752 family integrating conjugative element protein	-
A6R75_RS21650	4598178..4599089	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
A6R75_RS21655	4599086..4599778	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
A6R75_RS21660	4599775..4600173	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
A6R75_RS21665	4600185..4600544	-	360	WP_023980178.1	TIGR03745 family integrating conjugative element membrane protein	-
A6R75_RS21670	4600561..4600794	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
A6R75_RS21675	4600791..4601174	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
A6R75_RS21680	4601378..4601848	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
A6R75_RS21685	4601845..4602420	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
A6R75_RS21690	4602438..4603352	+	915	WP_003105629.1	AAA family ATPase	-
A6R75_RS21695	4603349..4603819	+	471	WP_003105626.1	hypothetical protein	-
A6R75_RS21700	4603816..4604316	+	501	WP_003090159.1	hypothetical protein	-
A6R75_RS21705	4604316..4605218	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
A6R75_RS21710	4605257..4605982	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4551215..4650171	98956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T62941 WP_003109777.1 NZ_CP015377:4601845-4602420 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T62941 NZ_CP015377:4601845-4602420 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT62941 WP_003050245.1 NZ_CP015377:4601378-4601848 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT62941 NZ_CP015377:4601378-4601848 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68