Detailed information of TA system TA062936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4088549..4089185
• Replicon: chromosome
• Accession: NZ_CP015375
• Organism: Bacillus subtilis subsp. subtilis strain KCTC 3135

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AS891_RS21570
• Protein ID: WP_003156187.1
• Coordinates: 4088549..4088899 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: AS891_RS21575
• Protein ID: WP_003225183.1
• Coordinates: 4088904..4089185 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS891_RS21530	4083593..4084192	-	600	WP_003246608.1	phosphoserine phosphatase RsbX	-
AS891_RS21535	4084192..4084980	-	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
AS891_RS21540	4084946..4085428	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
AS891_RS21545	4085425..4085754	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
AS891_RS21550	4085816..4086823	-	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
AS891_RS21555	4086835..4087236	-	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
AS891_RS21560	4087240..4087605	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
AS891_RS21565	4087610..4088434	-	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
AS891_RS21570	4088549..4088899	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AS891_RS21575	4088904..4089185	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AS891_RS21580	4089301..4090470	-	1170	WP_003234284.1	alanine racemase	-
AS891_RS21585	4090585..4091601	-	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
AS891_RS21590	4091767..4092132	-	366	WP_003234281.1	holo-ACP synthase	-
AS891_RS21595	4092227..4092826	+	600	WP_003246687.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T62936 WP_003156187.1 NZ_CP015375:c4088899-4088549 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T62936 NZ_CP015375:c4088899-4088549 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT62936 WP_003225183.1 NZ_CP015375:c4089185-4088904 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT62936 NZ_CP015375:c4089185-4088904 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32