Detailed information of TA system TA062909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 23109..23661
• Replicon: plasmid CBMB27-p2
• Accession: NZ_CP015369
• Organism: Methylobacterium phyllosphaerae strain CBMB27

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A088B2I3
• Locus tag: MCBMB27_RS28435
• Protein ID: WP_075382243.1
• Coordinates: 23371..23661 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: MCBMB27_RS28430
• Protein ID: WP_075382242.1
• Coordinates: 23109..23378 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCBMB27_RS28415	19215..20810	-	1596	WP_083683997.1	restriction endonuclease	-
MCBMB27_RS28420	20917..21429	+	513	WP_075382240.1	hypothetical protein	-
MCBMB27_RS28425	22213..22872	-	660	WP_075382241.1	ParA family protein	-
MCBMB27_RS28430	23109..23378	+	270	WP_075382242.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
MCBMB27_RS28435	23371..23661	+	291	WP_075382243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCBMB27_RS28440	24147..24962	+	816	WP_075382244.1	helix-turn-helix domain-containing protein	-
MCBMB27_RS28445	25137..25358	+	222	WP_075382245.1	hypothetical protein	-
MCBMB27_RS28450	27498..28493	-	996	WP_075382246.1	DUF4424 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52290	52290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• Antitoxin ParD (1-83)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10585.08 Da
• Isoelectric Point: 7.5696

>T62909 WP_075382243.1 NZ_CP015369:23371-23661 [Methylobacterium phyllosphaerae]
MSDYTLTRRAEADLFDIAVHGYRQFGVRQAEAYAAGLEHAFQLLADNPGMGRKADAIRPGVHRHEHAAHVILYEVAADGV
LILAIVHGRSVRRLAL

Nucleotide

• Length: 291 bp

>T62909 NZ_CP015369:23371-23661 [Methylobacterium phyllosphaerae]
ATGAGTGACTATACGCTCACGAGGAGGGCCGAGGCGGACCTCTTCGACATCGCCGTCCACGGCTACCGGCAGTTCGGTGT
CCGCCAAGCCGAAGCCTACGCTGCCGGTCTGGAGCACGCCTTCCAGCTTCTCGCCGACAACCCCGGCATGGGCCGAAAGG
CCGACGCGATCCGCCCAGGCGTGCACCGGCACGAGCATGCCGCGCACGTCATCCTGTACGAGGTAGCGGCTGACGGCGTC
CTGATCCTAGCCATCGTCCACGGCCGCAGCGTGAGGCGGCTGGCCCTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10312.67 Da
• Isoelectric Point: 7.2061

>AT62909 WP_075382242.1 NZ_CP015369:23109-23378 [Methylobacterium phyllosphaerae]
MATMTISLPDPMKEWIEAQIRQGDFASTSDYVRDLVRRDRERRAHPELTLEDLRRIVDDARASGPSRRKVPEILARAKKH
AQADQMPDE

Nucleotide

• Length: 270 bp

>AT62909 NZ_CP015369:23109-23378 [Methylobacterium phyllosphaerae]
ATGGCGACCATGACCATCTCTCTGCCCGACCCGATGAAGGAGTGGATCGAGGCGCAGATCAGGCAGGGCGATTTCGCCAG
CACGAGCGACTATGTCCGCGATCTCGTCCGCCGCGATCGGGAGCGGCGTGCCCATCCCGAACTGACGCTCGAGGATCTGC
GCCGGATCGTCGATGACGCGCGCGCCAGCGGACCGAGCCGGCGCAAGGTCCCTGAGATCCTCGCACGGGCGAAGAAGCAC
GCGCAGGCCGATCAGATGCCGGATGAGTGA

Structures

Toxin

• AlphaFold DB: A0A088B2I3