Detailed information of TA system TA062903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2478362..2479340
• Replicon: chromosome
• Accession: NZ_CP015350
• Organism: Bacillus thuringiensis strain MYBT18246

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BT246_RS12865
• Protein ID: WP_065483612.1
• Coordinates: 2478362..2479099 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BT246_RS12870
• Protein ID: WP_000237818.1
• Coordinates: 2479212..2479340 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT246_RS12845	2474004..2474786	+	783	WP_065483606.1	class I SAM-dependent methyltransferase	-
BT246_RS12850	2474944..2476620	-	1677	WP_141550274.1	alpha-keto acid decarboxylase family protein	-
BT246_RS12855	2476737..2477219	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
BT246_RS12860	2477386..2478123	+	738	WP_053564492.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BT246_RS12865	2478362..2479099	+	738	WP_065483612.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BT246_RS12870	2479212..2479340	+	129	WP_000237818.1	hypothetical protein	Antitoxin
BT246_RS12875	2479413..2479589	+	177	WP_065483615.1	stage II sporulation protein SB	-
BT246_RS12880	2479606..2479995	-	390	WP_065483618.1	YxeA family protein	-
BT246_RS12885	2480207..2481676	+	1470	WP_065483622.1	beta-Ala-His dipeptidase	-
BT246_RS12890	2481922..2482731	+	810	WP_065483624.1	C39 family peptidase	-
BT246_RS12895	2482757..2483359	+	603	WP_065483627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2455294..2488392	33098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28310.03 Da
• Isoelectric Point: 8.2998

>T62903 WP_065483612.1 NZ_CP015350:2478362-2479099 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKTNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T62903 NZ_CP015350:2478362-2479099 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAACTAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT62903 WP_000237818.1 NZ_CP015350:2479212-2479340 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT62903 NZ_CP015350:2479212-2479340 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5