Detailed information of TA system TA062900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4048341..4049912
• Replicon: chromosome
• Accession: NZ_CP015347
• Organism: Proteus mirabilis strain AOUC-001

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B4F0T8
• Locus tag: AOUC001_RS19025
• Protein ID: WP_012368637.1
• Coordinates: 4048599..4049912 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: AOUC001_RS19020
• Protein ID: WP_004249771.1
• Coordinates: 4048341..4048598 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOUC001_RS19000	4044303..4044446	+	144	WP_004246509.1	50S ribosomal protein L34	-
AOUC001_RS19005	4044464..4044823	+	360	WP_004246510.1	ribonuclease P protein component	-
AOUC001_RS20710	4044787..4045047	+	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
AOUC001_RS19010	4045050..4046690	+	1641	WP_004246512.1	membrane protein insertase YidC	-
AOUC001_RS19015	4046828..4048192	+	1365	WP_004246513.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
AOUC001_RS19020	4048341..4048598	+	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
AOUC001_RS19025	4048599..4049912	+	1314	WP_012368637.1	type II toxin-antitoxin system HipA family toxin	Toxin
AOUC001_RS19030	4050315..4050779	+	465	WP_004249773.1	DUF1097 domain-containing protein	-
AOUC001_RS19035	4050964..4051893	+	930	WP_004246518.1	metallophosphoesterase	-
AOUC001_RS19040	4051925..4053886	+	1962	WP_012368635.1	hypothetical protein	-
AOUC001_RS19045	4053940..4054743	-	804	WP_004249775.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49670.78 Da
• Isoelectric Point: 6.5023

>T62900 WP_012368637.1 NZ_CP015347:4048599-4049912 [Proteus mirabilis]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T62900 NZ_CP015347:4048599-4049912 [Proteus mirabilis]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATGTGGGCATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTTTCGATGCCATTACAGCTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTGTTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGCGGCTATTGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGGATATCAAAAAAATAGAATATCAAAAACTCAATGATATTGAGCTTGAAAAAATTCTACTAGGATATA
AATCCAATACTCCACTCGGTATGCTTAAAGAATATACTGATTTCAGAATTTCCATCGCTGGAGCACAAGAAAAAACAGCA
TTACTTTTAAAAGATAATCATTGGTGTGTGCCACATAATAACACACCAACTACCCATATTATTAAACTGCCAATTGGTGT
TATTGAAAGTCATTCTTATACAATGGATTTATCCAATAGTGTAGAAAATGAGTATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCCGTCCCCCACTGCTCTATTATTAAAACAAAAAATGTTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TATTCATCTGATAAATCGTGGATAATACGTTTACCTCAAGAAGATTTTTGCCAAATTTTAAATATACCTTCTGCGCTAAA
ATATGAAAATGAAGGAGGGCCTGGCATTATCGATATTATGAAATATTTACTTGGCTCATCAAATGCCGATCAAGATCGTT
TTCATTTTATGAAGGCACAAGTGCTATTTTGGCTTTTGGCTGCAACGGATGGACACGCAAAGAATTTTTCTGTTTTTATC
AACCAAGAAGGACGTTTCCATCTCACTCCCCTTTATGACATTATGTCCATCTATCCCAATTTCGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTTGCTATGGGATTAAAAGCAAGCCGAGGCAAAAAATACGCAATAGAACAAATTTTTCCTCGTC
ATTTCTACCAAACAGCTGAAGCTGTCGGTTTCGATCCTTCATTAATGAGCGATATTCTTCGTTATTTTTATCAAGAAATG
GACGATGTCATACAACGCGTAAAACAGCAATTACCCGAGGATTTCCCTTCTGAGATTAGCAATACAATATTAGATGGAAT
GAAAGCACGCGCTGTTAGATTAGCTAACATTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT62900 WP_004249771.1 NZ_CP015347:4048341-4048598 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT62900 NZ_CP015347:4048341-4048598 [Proteus mirabilis]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTGTCCAATTTTGAACTTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: B4F0T8

Antitoxin

• AlphaFold DB: B4F0T9