Detailed information of TA system TA062893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 13097..13740
• Replicon: chromosome
• Accession: NZ_CP015347
• Organism: Proteus mirabilis strain AOUC-001

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AOUC001_RS00100
• Protein ID: WP_001044770.1
• Coordinates: 13097..13513 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AOUC001_RS00105
• Protein ID: WP_001261282.1
• Coordinates: 13510..13740 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOUC001_RS00065	8639..8875	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
AOUC001_RS00070	8872..9234	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
AOUC001_RS00075	9252..10946	-	1695	WP_000105636.1	mercury(II) reductase	-
AOUC001_RS20175	10998..11420	-	423	WP_001340589.1	organomercurial transporter MerC	-
AOUC001_RS20180	11456..11566	-	111	Protein_11	mercuric transport protein periplasmic component	-
AOUC001_RS00085	11582..12279	-	698	Protein_12	IS1 family transposase	-
AOUC001_RS00095	12313..13023	-	711	WP_004152334.1	ATP-binding protein	-
AOUC001_RS00100	13097..13513	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOUC001_RS00105	13510..13740	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AOUC001_RS21470	13697..14158	+	462	WP_072202616.1	hypothetical protein	-
AOUC001_RS00110	14302..14652	+	351	WP_000493378.1	hypothetical protein	-
AOUC001_RS00115	14703..15446	+	744	WP_000129823.1	hypothetical protein	-
AOUC001_RS00120	15443..16219	+	777	WP_000015958.1	site-specific integrase	-
AOUC001_RS00125	16277..16534	-	258	WP_000764642.1	hypothetical protein	-
AOUC001_RS21475	16663..16767	-	105	WP_032409716.1	hypothetical protein	-
AOUC001_RS00130	17302..18168	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	12004..12279	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T62893 WP_001044770.1 NZ_CP015347:c13513-13097 [Proteus mirabilis]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T62893 NZ_CP015347:c13513-13097 [Proteus mirabilis]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT62893 WP_001261282.1 NZ_CP015347:c13740-13510 [Proteus mirabilis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT62893 NZ_CP015347:c13740-13510 [Proteus mirabilis]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3