Detailed information of TA system TA062879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 753859..754502
• Replicon: plasmid pM0123d
• Accession: NZ_CP015322
• Organism: Mesorhizobium amorphae CCNWGS0123

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G6YG92
• Locus tag: A6B35_RS33260
• Protein ID: WP_006204729.1
• Coordinates: 754116..754502 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A6B35_RS33255
• Protein ID: WP_040583545.1
• Coordinates: 753859..754116 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6B35_RS33235	749358..750494	-	1137	WP_006204722.1	DNA phosphorothioation system sulfurtransferase DndC	-
A6B35_RS33240	750491..751639	-	1149	WP_006204723.1	cysteine desulfurase	-
A6B35_RS33245	751947..752663	-	717	WP_040583543.1	SMC-Scp complex subunit ScpB	-
A6B35_RS33250	752669..753689	-	1021	Protein_644	DUF1403 family protein	-
A6B35_RS33255	753859..754116	+	258	WP_040583545.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
A6B35_RS33260	754116..754502	+	387	WP_006204729.1	type II toxin-antitoxin system VapC family toxin	Toxin
A6B35_RS33265	754499..755644	+	1146	WP_050900819.1	site-specific integrase	-
A6B35_RS33270	755720..757128	+	1409	Protein_648	Fic family protein	-
A6B35_RS33280	757837..759039	+	1203	WP_006204733.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..948568	948568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (2-84)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14024.25 Da
• Isoelectric Point: 5.1447

>T62879 WP_006204729.1 NZ_CP015322:754116-754502 [Mesorhizobium amorphae CCNWGS0123]
MVIDTSAIVAILRSEPEAARLERALISDPIRLVPATCVLEARMVMVSRRGEHALAEIDLWLHKIAVNIIPVDSELVDLAT
QAWLSYGKGHHPAALNFADCFSYALAKRSDEPLLFIGNDFSKTDVVAA

Nucleotide

• Length: 387 bp

>T62879 NZ_CP015322:754116-754502 [Mesorhizobium amorphae CCNWGS0123]
ATGGTGATCGACACCTCAGCCATTGTGGCGATCCTGCGTAGTGAACCCGAGGCGGCAAGGCTTGAAAGAGCACTTATCTC
CGATCCAATCCGTCTGGTTCCGGCGACCTGTGTACTCGAAGCACGTATGGTGATGGTCAGCAGGCGCGGCGAACACGCAT
TGGCCGAGATCGATCTTTGGCTTCATAAGATCGCTGTCAACATCATTCCCGTTGACTCCGAATTGGTTGATCTGGCCACC
CAAGCTTGGCTCTCCTACGGCAAGGGCCATCATCCGGCCGCCCTGAACTTTGCCGATTGCTTCTCCTACGCATTGGCAAA
ACGTTCGGACGAGCCGCTGCTGTTCATCGGTAATGACTTTTCCAAGACAGATGTGGTGGCAGCATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9539.76 Da
• Isoelectric Point: 6.7276

>AT62879 WP_040583545.1 NZ_CP015322:753859-754116 [Mesorhizobium amorphae CCNWGS0123]
VSLNIKNPATVALAEELARRQGISKTAAIHQALSERLHRMGFGDAAQERLLAELRAIRERLARLPDRDNRTDEEIIGYDE
HGIPN

Nucleotide

• Length: 258 bp

>AT62879 NZ_CP015322:753859-754116 [Mesorhizobium amorphae CCNWGS0123]
GTGTCCCTCAACATCAAAAATCCGGCAACCGTCGCCCTCGCCGAGGAGCTCGCACGCCGCCAGGGTATCAGCAAGACTGC
GGCCATTCATCAGGCCCTGAGCGAGCGCCTGCATCGAATGGGTTTCGGTGACGCTGCGCAGGAGCGTCTGCTCGCGGAAC
TGCGCGCGATCCGGGAGAGACTGGCGCGGTTGCCTGACCGGGATAATCGCACCGACGAGGAGATCATCGGGTACGACGAA
CACGGAATTCCGAACTGA

Structures

Toxin

• AlphaFold DB: G6YG92