Detailed information of TA system TA062878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 3310767..3311361
• Replicon: chromosome
• Accession: NZ_CP015318
• Organism: Mesorhizobium amorphae CCNWGS0123

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: G6Y5B3
• Locus tag: A6B35_RS15515
• Protein ID: WP_006200559.1
• Coordinates: 3311173..3311361 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G6Y5B2
• Locus tag: A6B35_RS15510
• Protein ID: WP_006200558.1
• Coordinates: 3310767..3311168 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6B35_RS15485	3306173..3306793	-	621	WP_100084748.1	methyltransferase domain-containing protein	-
A6B35_RS15490	3306898..3308610	+	1713	Protein_3105	phage tail protein	-
A6B35_RS15495	3308694..3309017	+	324	WP_006200553.1	hypothetical protein	-
A6B35_RS15500	3309475..3309903	+	429	WP_006200556.1	hypothetical protein	-
A6B35_RS15505	3310247..3310564	-	318	WP_006200557.1	glycine zipper 2TM domain-containing protein	-
A6B35_RS15510	3310767..3311168	-	402	WP_006200558.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A6B35_RS15515	3311173..3311361	-	189	WP_006200559.1	type II toxin-antitoxin system HicA family toxin	Toxin
A6B35_RS15520	3311470..3312039	+	570	WP_006200560.1	hypothetical protein	-
A6B35_RS15525	3312243..3313088	+	846	WP_006200542.1	hypothetical protein	-
A6B35_RS15530	3313273..3314262	+	990	WP_065518212.1	Coat protein	-
A6B35_RS15540	3315204..3315803	+	600	WP_006200538.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3306898..3327162	20264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6932.07 Da
• Isoelectric Point: 11.3897

>T62878 WP_006200559.1 NZ_CP015318:c3311361-3311173 [Mesorhizobium amorphae CCNWGS0123]
MKSADVIDRLLADGWFEVARKGSHAQFKHASKPGRVTVPHPRRDIPIGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T62878 NZ_CP015318:c3311361-3311173 [Mesorhizobium amorphae CCNWGS0123]
ATGAAGAGCGCCGATGTCATCGACAGACTGTTGGCTGATGGCTGGTTCGAGGTGGCTCGGAAGGGCAGTCACGCTCAGTT
CAAGCACGCGTCGAAACCCGGCCGGGTGACGGTTCCGCATCCCAGGCGCGACATCCCGATCGGCACGTTGAAGAGCATAG
AAAAGCAATCCGGTCTGAAGCTGAGGTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14313.08 Da
• Isoelectric Point: 4.4693

>AT62878 WP_006200558.1 NZ_CP015318:c3311168-3310767 [Mesorhizobium amorphae CCNWGS0123]
MRHYIGLIHKEADSDFGVSFPDFPGVVTAGTSLDDARGMAEEALAFHVEGLVEDGEAIPEPSSLEEVMADKDNLTGVAIL
VALKTDATKVVRVNVTIPEDVLHQIDQYAERHGYSRSGFLTAAAKKAMRTEAA

Nucleotide

• Length: 402 bp

>AT62878 NZ_CP015318:c3311168-3310767 [Mesorhizobium amorphae CCNWGS0123]
ATGCGTCACTATATCGGACTGATCCACAAGGAAGCGGACAGCGACTTCGGCGTATCCTTTCCGGACTTTCCGGGCGTGGT
CACGGCCGGCACCAGCCTGGACGACGCACGCGGCATGGCGGAGGAGGCACTGGCCTTCCACGTCGAGGGCCTTGTCGAGG
ATGGTGAAGCCATTCCCGAACCGTCTTCCCTCGAAGAGGTGATGGCCGACAAGGACAACCTGACGGGCGTCGCCATACTG
GTCGCCTTGAAGACCGATGCGACGAAGGTGGTGCGCGTCAACGTCACCATTCCGGAGGACGTCCTTCACCAGATCGATCA
GTACGCGGAACGCCACGGCTACAGCCGCTCCGGATTCCTGACCGCGGCCGCAAAAAAGGCGATGCGGACCGAAGCGGCGT
AG

Structures

Toxin

• AlphaFold DB: G6Y5B3

Antitoxin

• AlphaFold DB: G6Y5B2