Detailed information of TA system TA062877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3293731..3294320
• Replicon: chromosome
• Accession: NZ_CP015318
• Organism: Mesorhizobium amorphae CCNWGS0123

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G6Y886
• Locus tag: A6B35_RS15415
• Protein ID: WP_006201666.1
• Coordinates: 3293731..3294084 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A6B35_RS35580
• Protein ID: WP_006201665.1
• Coordinates: 3294078..3294320 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6B35_RS35575	3288874..3289296	-	423	WP_156788620.1	hypothetical protein	-
A6B35_RS15385	3289688..3290158	-	471	WP_006205693.1	Lrp/AsnC family transcriptional regulator	-
A6B35_RS15390	3290315..3291067	+	753	WP_065518210.1	tryptophan 2,3-dioxygenase	-
A6B35_RS15395	3291388..3291810	+	423	WP_006201671.1	DoxX family protein	-
A6B35_RS15400	3291841..3292146	-	306	WP_006201670.1	hypothetical protein	-
A6B35_RS15405	3292244..3292513	-	270	WP_006201669.1	hypothetical protein	-
A6B35_RS15410	3292720..3293322	-	603	WP_006201668.1	hypothetical protein	-
A6B35_RS15415	3293731..3294084	-	354	WP_006201666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A6B35_RS35580	3294078..3294320	-	243	WP_006201665.1	hypothetical protein	Antitoxin
A6B35_RS15420	3294468..3295448	-	981	WP_006201664.1	IS1595 family transposase	-
A6B35_RS15425	3295743..3295976	+	234	WP_006201663.1	hypothetical protein	-
A6B35_RS15430	3296584..3297042	+	459	WP_006201662.1	hypothetical protein	-
A6B35_RS34520	3297035..3297340	+	306	Protein_3093	hypothetical protein	-
A6B35_RS15435	3297288..3298103	+	816	WP_040582586.1	terminase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3294468..3295448	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-112)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12959.73 Da
• Isoelectric Point: 4.6027

>T62877 WP_006201666.1 NZ_CP015318:c3294084-3293731 [Mesorhizobium amorphae CCNWGS0123]
MVDDVAYQPDRGDFIYLDFDPQSGTEQAGRRPALVISPRDFNIATGLAAVCPITNTLTGSKFEVPVPRGVKLTGVIQSYQ
FRTLDWIARNASFHSKANEELMWEVVGRIEAILCIDG

Nucleotide

• Length: 354 bp

>T62877 NZ_CP015318:c3294084-3293731 [Mesorhizobium amorphae CCNWGS0123]
TTGGTAGACGACGTAGCCTATCAGCCTGACAGAGGCGATTTTATCTATCTGGATTTTGATCCGCAGTCAGGCACAGAGCA
GGCCGGTCGCCGGCCTGCTCTCGTCATTTCTCCTCGCGATTTTAACATCGCAACGGGCTTGGCAGCCGTTTGTCCGATCA
CCAACACGTTGACCGGGAGCAAGTTCGAGGTTCCGGTTCCTCGCGGCGTCAAACTGACTGGCGTCATTCAATCGTATCAG
TTCCGCACGCTGGACTGGATAGCGCGAAACGCTTCTTTCCATTCAAAAGCCAATGAAGAACTCATGTGGGAGGTCGTCGG
GCGCATTGAGGCAATTCTCTGCATCGACGGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9190.38 Da
• Isoelectric Point: 5.8573

>AT62877 WP_006201665.1 NZ_CP015318:c3294320-3294078 [Mesorhizobium amorphae CCNWGS0123]
MQTTISRWGNSLALRLPKHVTEEVRLVEGVCVNVEIEDGSIRVTPTRKRFQLSELLKGETRQETGNREVDWGQPKGDEVW

Nucleotide

• Length: 243 bp

>AT62877 NZ_CP015318:c3294320-3294078 [Mesorhizobium amorphae CCNWGS0123]
ATGCAAACAACGATCAGCAGGTGGGGCAATAGCCTCGCGCTGCGACTTCCAAAGCACGTGACAGAGGAGGTACGGCTGGT
CGAAGGAGTTTGCGTAAACGTTGAAATCGAAGACGGTTCAATCAGGGTAACACCGACACGCAAACGCTTTCAGCTTTCCG
AACTTCTGAAAGGCGAAACTCGTCAAGAGACTGGCAACCGTGAAGTTGATTGGGGTCAACCGAAGGGTGACGAAGTTTGG
TAG

Structures

Toxin

• AlphaFold DB: G6Y886