Detailed information of TA system TA062876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1196955..1197757
• Replicon: chromosome
• Accession: NZ_CP015318
• Organism: Mesorhizobium amorphae CCNWGS0123

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: G6YA82
• Locus tag: A6B35_RS05805
• Protein ID: WP_006202377.1
• Coordinates: 1197230..1197757 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: G6YA83
• Locus tag: A6B35_RS05800
• Protein ID: WP_006202378.1
• Coordinates: 1196955..1197233 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6B35_RS05775	1192885..1193292	-	408	WP_006202384.1	DUF2000 family protein	-
A6B35_RS05780	1193328..1194203	+	876	WP_040582804.1	AraC family transcriptional regulator	-
A6B35_RS05785	1194289..1194759	-	471	WP_040582816.1	MarR family winged helix-turn-helix transcriptional regulator	-
A6B35_RS05790	1194981..1195709	-	729	WP_006202380.1	intradiol ring-cleavage dioxygenase	-
A6B35_RS05795	1195814..1196872	+	1059	WP_006202379.1	GTP-binding protein	-
A6B35_RS05800	1196955..1197233	+	279	WP_006202378.1	DUF1778 domain-containing protein	Antitoxin
A6B35_RS05805	1197230..1197757	+	528	WP_006202377.1	hypothetical protein	Toxin
A6B35_RS05810	1197871..1198845	+	975	WP_006202376.1	WD40 repeat domain-containing protein	-
A6B35_RS05815	1198852..1199304	-	453	WP_006202375.1	hypothetical protein	-
A6B35_RS05820	1199461..1200843	+	1383	WP_006202374.1	aspartate aminotransferase family protein	-
A6B35_RS05825	1200840..1202261	+	1422	WP_050900736.1	glutamine synthetase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18835.50 Da
• Isoelectric Point: 8.2809

>T62876 WP_006202377.1 NZ_CP015318:1197230-1197757 [Mesorhizobium amorphae CCNWGS0123]
VTLPSWHEQPIAKQHDRASFDCGDAQMNDFLRRFARQSHEQNAAKTFCAIDDATTERILGFYTIAPSAVAYENVPVAMTK
GLARHEVSGFKLARLATDRSVAGQGLGGQLLAAAALRCLRLAGEGGGVLLIIDAKSERAARWYASYGAESLQGQSLTLVM
PLATFATELRAKGLL

Nucleotide

• Length: 528 bp

>T62876 NZ_CP015318:1197230-1197757 [Mesorhizobium amorphae CCNWGS0123]
GTGACACTTCCGTCCTGGCACGAGCAGCCGATTGCGAAACAGCACGATCGGGCCTCGTTCGACTGCGGTGATGCGCAGAT
GAACGATTTTCTGCGGCGGTTCGCGCGACAAAGCCATGAACAAAATGCCGCCAAGACCTTCTGTGCGATCGACGACGCGA
CTACCGAGCGGATTCTCGGCTTTTATACAATCGCGCCGTCTGCAGTAGCTTATGAAAACGTGCCCGTGGCCATGACGAAG
GGATTGGCGCGGCACGAGGTTTCCGGTTTCAAGCTCGCCCGTCTCGCGACCGATCGCAGCGTAGCAGGGCAAGGGCTGGG
CGGTCAGTTGCTTGCCGCCGCTGCGCTCCGCTGCTTGCGCCTCGCTGGCGAAGGTGGCGGCGTACTGCTCATCATTGACG
CCAAGAGTGAGCGGGCCGCCAGATGGTACGCCAGCTACGGTGCCGAGTCTCTTCAAGGCCAATCTCTGACGCTGGTGATG
CCGCTTGCGACTTTCGCGACGGAGCTGCGGGCAAAGGGGCTGCTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10106.73 Da
• Isoelectric Point: 9.8632

>AT62876 WP_006202378.1 NZ_CP015318:1196955-1197233 [Mesorhizobium amorphae CCNWGS0123]
MPRPADHKTERYQIRIAPAQKAMIARAAALSHQDVADFILNKVVPEAQAVIEAAEVTKVSDRNFTRILDLLENPPKPNAK
LRAAIAALPDTL

Nucleotide

• Length: 279 bp

>AT62876 NZ_CP015318:1196955-1197233 [Mesorhizobium amorphae CCNWGS0123]
ATGCCGCGGCCCGCCGACCACAAGACTGAACGTTATCAAATTCGCATTGCGCCCGCGCAGAAGGCGATGATCGCGCGCGC
CGCTGCACTCTCGCATCAGGACGTTGCCGACTTCATCCTCAACAAGGTCGTTCCTGAGGCACAGGCTGTGATCGAAGCCG
CAGAGGTCACAAAAGTATCCGACCGTAATTTCACACGTATACTCGACCTGCTGGAGAATCCGCCGAAACCCAATGCAAAA
CTGCGAGCGGCGATCGCGGCCCTGCCGGATACACTGTGA

Structures

Toxin

• AlphaFold DB: G6YA82

Antitoxin

• AlphaFold DB: G6YA83