Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240185..240827 Replicon chromosome
Accession NZ_CP015310
Organism Bacillus thuringiensis serovar morrisoni str. 4AA1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BTT_RS01355 Protein ID WP_000635965.1
Coordinates 240477..240827 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTT_RS01350 Protein ID WP_000004570.1
Coordinates 240185..240472 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTT_RS01325 (BTT_02660) 235502..236464 + 963 WP_016078970.1 UV DNA damage repair endonuclease UvsE -
BTT_RS01330 (BTT_02670) 236457..237029 - 573 WP_016078971.1 rhomboid family intramembrane serine protease -
BTT_RS01335 (BTT_02680) 237123..237482 + 360 WP_000583417.1 holo-ACP synthase -
BTT_RS01340 (BTT_02690) 237639..238589 + 951 WP_003306148.1 outer membrane lipoprotein carrier protein LolA -
BTT_RS01345 (BTT_02700) 238707..239876 + 1170 WP_000390595.1 alanine racemase -
BTT_RS01350 (BTT_02710) 240185..240472 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTT_RS01355 (BTT_02720) 240477..240827 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTT_RS01360 (BTT_02730) 240895..243063 + 2169 WP_016078972.1 Tex family protein -
BTT_RS01365 243121..243237 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BTT_RS01370 (BTT_02740) 243433..243891 + 459 WP_000343553.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T62874 WP_000635965.1 NZ_CP015310:240477-240827 [Bacillus thuringiensis serovar morrisoni str. 4AA1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T62874 NZ_CP015310:240477-240827 [Bacillus thuringiensis serovar morrisoni str. 4AA1]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT62874 WP_000004570.1 NZ_CP015310:240185-240472 [Bacillus thuringiensis serovar morrisoni str. 4AA1]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT62874 NZ_CP015310:240185-240472 [Bacillus thuringiensis serovar morrisoni str. 4AA1]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References