Detailed information of TA system TA062873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 57825..58462
• Replicon: plasmid d
• Accession: NZ_CP015292
• Organism: Cereibacter sphaeroides strain MBTLJ-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A3857_RS22545
• Protein ID: WP_011331362.1
• Coordinates: 58055..58462 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IV80
• Locus tag: A3857_RS22540
• Protein ID: WP_011331361.1
• Coordinates: 57825..58055 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3857_RS22520	53802..54689	-	888	WP_011331357.1	viral aspartic protease	-
A3857_RS22525	55252..55677	-	426	WP_011331358.1	helix-turn-helix transcriptional regulator	-
A3857_RS22530	55965..56390	+	426	WP_011331359.1	hypothetical protein	-
A3857_RS22535	56603..57436	+	834	WP_017140476.1	hypothetical protein	-
A3857_RS22540	57825..58055	+	231	WP_011331361.1	antitoxin	Antitoxin
A3857_RS22545	58055..58462	+	408	WP_011331362.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A3857_RS22550	58784..58942	+	159	WP_017140474.1	SDR family oxidoreductase	-
A3857_RS22555	58951..61575	+	2625	WP_017140473.1	cation-transporting P-type ATPase	-
A3857_RS22560	61607..61774	+	168	Protein_67	Hsp20/alpha crystallin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..105299	105299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14801.00 Da
• Isoelectric Point: 6.8714

>T62873 WP_011331362.1 NZ_CP015292:58055-58462 [Cereibacter sphaeroides]
MLRYMLDTNILIYTIRNRPGEVRAKFEAHHGTMCISAATAMELIYGAQKSQAVERNVAAVEGLLARLDILDLDLAAAQHA
GEIRAQLAQAGTPIGPFDVMIAGHARSKGLVLVSNNTREFERVDGLRLENWVQSS

Nucleotide

• Length: 408 bp

>T62873 NZ_CP015292:58055-58462 [Cereibacter sphaeroides]
ATGCTGCGCTACATGCTCGACACGAACATCCTGATCTACACGATCAGGAACCGGCCCGGCGAGGTCAGGGCAAAGTTCGA
GGCCCATCACGGGACCATGTGCATCTCGGCCGCAACCGCGATGGAACTGATATACGGTGCGCAGAAATCGCAGGCCGTCG
AGCGCAACGTGGCGGCGGTCGAGGGACTCCTCGCGCGCCTCGACATTCTGGATCTCGATCTGGCGGCTGCCCAGCATGCC
GGGGAGATCCGGGCGCAGTTGGCGCAGGCAGGGACGCCCATCGGGCCGTTCGACGTGATGATCGCGGGACACGCGCGATC
TAAGGGCCTGGTCCTCGTGAGCAACAATACGCGGGAATTCGAACGCGTCGACGGCCTGAGACTGGAGAACTGGGTTCAGT
CGTCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8777.77 Da
• Isoelectric Point: 4.5600

>AT62873 WP_011331361.1 NZ_CP015292:57825-58055 [Cereibacter sphaeroides]
VDRGSVFMNNRTQAIRLPKSVALPEGTRSVDIVRLGRAWLIVPSQDSWAQWFDEVSSSEDFMERRKQPDADERDDL

Nucleotide

• Length: 231 bp

>AT62873 NZ_CP015292:57825-58055 [Cereibacter sphaeroides]
GTGGATCGGGGATCGGTCTTCATGAACAACCGGACACAGGCCATCCGCCTGCCAAAATCAGTTGCCTTGCCGGAAGGCAC
GCGGAGCGTCGACATCGTCCGGCTGGGCAGAGCCTGGCTCATCGTGCCGAGCCAGGACAGCTGGGCCCAATGGTTCGATG
AGGTCTCTTCGAGTGAAGACTTCATGGAGCGCCGGAAGCAACCGGACGCCGATGAGCGCGACGATCTCTGA

Structures

Antitoxin

• AlphaFold DB: Q3IV80