Detailed information of TA system TA062827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 4319553..4320079
• Replicon: chromosome
• Accession: NZ_CP015278
• Organism: Mycobacterium intracellulare subsp. chimaera strain DSM 44623

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: MYCODSM44623_RS20075
• Protein ID: WP_008259048.1
• Coordinates: 4319777..4320079 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: MYCODSM44623_RS20070
• Protein ID: WP_008259047.1
• Coordinates: 4319553..4319780 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCODSM44623_RS20045	4314624..4315550	-	927	WP_042912749.1	MinD/ParA family protein	-
MYCODSM44623_RS20050	4315795..4316826	-	1032	WP_042912751.1	6-phosphofructokinase	-
MYCODSM44623_RS20055	4316870..4318363	-	1494	WP_042912754.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
MYCODSM44623_RS20060	4318360..4318659	-	300	WP_009952003.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
MYCODSM44623_RS20065	4318759..4319415	+	657	WP_087139897.1	amino acid-binding protein	-
MYCODSM44623_RS20070	4319553..4319780	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
MYCODSM44623_RS20075	4319777..4320079	+	303	WP_008259048.1	hypothetical protein	Toxin
MYCODSM44623_RS20080	4320195..4320503	-	309	WP_008259049.1	hypothetical protein	-
MYCODSM44623_RS20085	4320562..4323483	-	2922	WP_014712101.1	MMPL family transporter	-
MYCODSM44623_RS20090	4323480..4323884	-	405	WP_008259052.1	hypothetical protein	-
MYCODSM44623_RS20095	4324131..4324649	+	519	WP_042912757.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T62827 WP_008259048.1 NZ_CP015278:4319777-4320079 [Mycobacterium intracellulare subsp. chimaera]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T62827 NZ_CP015278:4319777-4320079 [Mycobacterium intracellulare subsp. chimaera]
ATGATCGCGCCGGGCGACATCGCGCCGCGGCGAGACACCCGCCGCGACCTCTACGTCGCGGTGCTCTCCAACGCGATCCA
CCTCGCGGCCAACACTGGTCGCGTCATCGTCTGCCCGTTCATCCCTGGGAAAATCCCCGACGACACGATGGCCATGGTCG
TTCCGCTCACGCAGCCCGAAGGCACTCTTCTCCCCGAACTGGTGCAGTGGCTGCCGACTTCGGCGCTCGGTGAACCGATC
GGGGCCGTGTCGGCGGCCGCGCTTAGCGAGGCGATGTCGATCGTGACCGCGCTGATCACGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT62827 WP_008259047.1 NZ_CP015278:4319553-4319780 [Mycobacterium intracellulare subsp. chimaera]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT62827 NZ_CP015278:4319553-4319780 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAAGAGAAGATTTCTGTCACCGTCGACGCCGCCGTGCTCGCCGACGCCGACTCCGACGCCAAAGCGGCCGGACT
GAATCGCTCCGAGATGATCGAGCGGGCGCTGCGCAATGAGCATCTACGTCGCGCGCTTCATACCTATACGACGCGGACCG
CACCAGCGCTCAACATCGACTCCTACGCCGGGCAAGTGTATGAAGCCAACCGGGCCGCGGACCTATGA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0