Detailed information of TA system TA062821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1265858..1266511
• Replicon: chromosome
• Accession: NZ_CP015278
• Organism: Mycobacterium intracellulare subsp. chimaera strain DSM 44623

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MYCODSM44623_RS06290
• Protein ID: WP_008253911.1
• Coordinates: 1266050..1266511 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: MYCODSM44623_RS06285
• Protein ID: WP_009957321.1
• Coordinates: 1265858..1266046 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCODSM44623_RS06275	1261831..1263408	+	1578	WP_042911067.1	serine hydrolase	-
MYCODSM44623_RS06280	1263405..1265792	+	2388	WP_042910814.1	cation-translocating P-type ATPase	-
MYCODSM44623_RS06285	1265858..1266046	+	189	WP_009957321.1	antitoxin	Antitoxin
MYCODSM44623_RS06290	1266050..1266511	+	462	WP_008253911.1	SRPBCC family protein	Toxin
MYCODSM44623_RS06295	1266598..1267368	+	771	WP_042910815.1	VOC family protein	-
MYCODSM44623_RS06300	1267442..1267876	+	435	WP_008253914.1	hypothetical protein	-
MYCODSM44623_RS06305	1267881..1268369	-	489	WP_036454690.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
MYCODSM44623_RS06310	1268369..1269883	-	1515	WP_042910816.1	carotenoid oxygenase family protein	-
MYCODSM44623_RS06315	1269886..1271106	-	1221	WP_042910817.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T62821 WP_008253911.1 NZ_CP015278:1266050-1266511 [Mycobacterium intracellulare subsp. chimaera]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T62821 NZ_CP015278:1266050-1266511 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACTATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT62821 WP_009957321.1 NZ_CP015278:1265858-1266046 [Mycobacterium intracellulare subsp. chimaera]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT62821 NZ_CP015278:1265858-1266046 [Mycobacterium intracellulare subsp. chimaera]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCGGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X8A187