Detailed information of TA system TA062813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 4609223..4609749
• Replicon: chromosome
• Accession: NZ_CP015272
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-1

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: MYCOZU1_RS21365
• Protein ID: WP_008259048.1
• Coordinates: 4609447..4609749 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: MYCOZU1_RS21360
• Protein ID: WP_008259047.1
• Coordinates: 4609223..4609450 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU1_RS21335	4604294..4605220	-	927	WP_042912749.1	MinD/ParA family protein	-
MYCOZU1_RS21340	4605465..4606496	-	1032	WP_042912751.1	6-phosphofructokinase	-
MYCOZU1_RS21345	4606540..4608033	-	1494	WP_042912754.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
MYCOZU1_RS21350	4608030..4608329	-	300	WP_009952003.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
MYCOZU1_RS21355	4608429..4609085	+	657	WP_087139897.1	amino acid-binding protein	-
MYCOZU1_RS21360	4609223..4609450	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
MYCOZU1_RS21365	4609447..4609749	+	303	WP_008259048.1	hypothetical protein	Toxin
MYCOZU1_RS21370	4609865..4610173	-	309	WP_008259049.1	hypothetical protein	-
MYCOZU1_RS21375	4610232..4613153	-	2922	WP_014712101.1	MMPL family transporter	-
MYCOZU1_RS21380	4613150..4613554	-	405	WP_008259052.1	hypothetical protein	-
MYCOZU1_RS21385	4613801..4614319	+	519	WP_042912757.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T62813 WP_008259048.1 NZ_CP015272:4609447-4609749 [Mycobacterium intracellulare subsp. chimaera]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T62813 NZ_CP015272:4609447-4609749 [Mycobacterium intracellulare subsp. chimaera]
ATGATCGCGCCGGGCGACATCGCGCCGCGGCGAGACACCCGCCGCGACCTCTACGTCGCGGTGCTCTCCAACGCGATCCA
CCTCGCGGCCAACACTGGTCGCGTCATCGTCTGCCCGTTCATCCCTGGGAAAATCCCCGACGACACGATGGCCATGGTCG
TTCCGCTCACGCAGCCCGAAGGCACTCTTCTCCCCGAACTGGTGCAGTGGCTGCCGACTTCGGCGCTCGGTGAACCGATC
GGGGCCGTGTCGGCGGCCGCGCTTAGCGAGGCGATGTCGATCGTGACCGCGCTGATCACGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT62813 WP_008259047.1 NZ_CP015272:4609223-4609450 [Mycobacterium intracellulare subsp. chimaera]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT62813 NZ_CP015272:4609223-4609450 [Mycobacterium intracellulare subsp. chimaera]
ATGGCCAAAGAGAAGATTTCTGTCACCGTCGACGCCGCCGTGCTCGCCGACGCCGACTCCGACGCCAAAGCGGCCGGACT
GAATCGCTCCGAGATGATCGAGCGGGCGCTGCGCAATGAGCATCTACGTCGCGCGCTTCATACCTATACGACGCGGACCG
CACCAGCGCTCAACATCGACTCCTACGCCGGGCAAGTGTATGAAGCCAACCGGGCCGCGGACCTATGA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0