Detailed information of TA system TA062805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 1072155..1072822
• Replicon: chromosome
• Accession: NZ_CP015272
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5PE24
• Locus tag: MYCOZU1_RS05280
• Protein ID: WP_007168407.1
• Coordinates: 1072430..1072822 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D5PE23
• Locus tag: MYCOZU1_RS05275
• Protein ID: WP_007168406.1
• Coordinates: 1072155..1072430 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU1_RS30505	1067659..1067985	-	327	WP_033711234.1	hypothetical protein	-
MYCOZU1_RS05250	1067975..1068208	-	234	Protein_1032	transposase	-
MYCOZU1_RS05255	1068226..1069437	-	1212	Protein_1033	ISL3 family transposase	-
MYCOZU1_RS05260	1069576..1070208	-	633	Protein_1034	DDE-type integrase/transposase/recombinase	-
MYCOZU1_RS30015	1070160..1070300	-	141	WP_145958858.1	SDR family oxidoreductase	-
MYCOZU1_RS05265	1070344..1071047	-	704	Protein_1036	histidine phosphatase family protein	-
MYCOZU1_RS05270	1071130..1072002	+	873	WP_007168405.1	MaoC family dehydratase N-terminal domain-containing protein	-
MYCOZU1_RS05275	1072155..1072430	+	276	WP_007168406.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCOZU1_RS05280	1072430..1072822	+	393	WP_007168407.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCOZU1_RS05285	1072970..1074129	-	1160	Protein_1040	trehalose-phosphatase	-
MYCOZU1_RS05290	1074171..1075509	-	1339	Protein_1041	DUF1298 domain-containing protein	-
MYCOZU1_RS30020	1075771..1075893	-	123	Protein_1042	IS1595 family transposase	-
MYCOZU1_RS05300	1076229..1077563	+	1335	Protein_1043	IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-45)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14305.73 Da
• Isoelectric Point: 10.5032

>T62805 WP_007168407.1 NZ_CP015272:1072430-1072822 [Mycobacterium intracellulare subsp. chimaera]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRLPRVSSALARTEVMRALLHKGESARRAGRRALASLDLLRIDNRLLDLAG
GLLPIELRTLDAIHLATAQRLGMDLGRLCTYDDRMRDAAEALGMAVIAPS

Nucleotide

• Length: 393 bp

>T62805 NZ_CP015272:1072430-1072822 [Mycobacterium intracellulare subsp. chimaera]
ATGGCGGCCATCTACCTGGACTCGTCCGCAATCGTCAAGCTTGCAGTTCGTGAGCCAGAGTCGGATGCGCTGCGTCGCTA
CCTTCGCACCCGGCTGCCGCGGGTGTCGAGTGCCCTTGCGCGGACGGAGGTCATGCGGGCTCTGCTCCACAAGGGTGAAT
CAGCCCGAAGAGCCGGACGCCGGGCGCTCGCGAGCCTAGACCTACTACGCATCGACAACCGATTGCTCGATCTTGCTGGT
GGGCTGCTGCCAATCGAGCTACGCACCCTCGATGCAATTCACCTGGCGACTGCCCAGCGGCTCGGCATGGATCTTGGCCG
GTTGTGCACTTACGACGACCGCATGCGCGACGCCGCCGAGGCGCTCGGCATGGCAGTGATTGCCCCGTCTTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10150.63 Da
• Isoelectric Point: 5.1881

>AT62805 WP_007168406.1 NZ_CP015272:1072155-1072430 [Mycobacterium intracellulare subsp. chimaera]
MSTVGVRELRQRASELLRRVEAGETIEITDRGRPIALLSPMPEGGPYQQMLASGGIERATLDFDDLPEPVRLKTGIELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT62805 NZ_CP015272:1072155-1072430 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTACGGTGGGCGTTCGTGAGCTGCGGCAGCGGGCCAGCGAACTCCTCCGTCGGGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGTGGTCGGCCTATCGCCCTTCTCTCGCCGATGCCGGAGGGCGGTCCGTACCAGCAGATGCTGGCAAGCG
GCGGGATCGAACGTGCGACGCTTGACTTTGATGACCTTCCCGAGCCGGTCCGACTCAAAACGGGTATCGAGCTGCCGTCC
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A1X1XV84

Antitoxin

• AlphaFold DB: A0A1X1XUZ3