Detailed information of TA system TA062803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 287949..288847
• Replicon: chromosome
• Accession: NZ_CP015272
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-1

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: A0A7I9ZD82
• Locus tag: MYCOZU1_RS01395
• Protein ID: WP_042910420.1
• Coordinates: 287949..288509 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: A0A7I9ZDJ0
• Locus tag: MYCOZU1_RS01400
• Protein ID: WP_042910421.1
• Coordinates: 288506..288847 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU1_RS01375	284829..285314	+	486	WP_051445817.1	hypothetical protein	-
MYCOZU1_RS29885	285586..285822	+	237	WP_142383091.1	hypothetical protein	-
MYCOZU1_RS01385	286482..286664	-	183	WP_042910451.1	antitoxin VbhA family protein	-
MYCOZU1_RS01390	286768..287772	-	1005	WP_042910419.1	tyrosine-type recombinase/integrase	-
MYCOZU1_RS01395	287949..288509	-	561	WP_042910420.1	RES domain-containing protein	Toxin
MYCOZU1_RS01400	288506..288847	-	342	WP_042910421.1	DUF2384 domain-containing protein	Antitoxin
MYCOZU1_RS01405	288934..290087	+	1154	Protein_274	hypothetical protein	-
MYCOZU1_RS01410	290265..291259	-	995	Protein_275	IS481 family transposase	-
MYCOZU1_RS01415	292295..292927	-	633	WP_042910422.1	CAP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20501.00 Da
• Isoelectric Point: 5.8540

>T62803 WP_042910420.1 NZ_CP015272:c288509-287949 [Mycobacterium intracellulare subsp. chimaera]
VSDALDEGLVQRIDACGTTPWSGTCYRYTTAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAKACMVEVERAANAASTT
AIKMLEAAYRLHHIQVEELNVLDLRTPESRDAVGLDDDDVYGDDWSACQAVGHTAWFLHMQGILVPAAGGIGLVISAYEQ
RTRPRQLHLRHSENLTPALYTQYRSS

Nucleotide

• Length: 561 bp

>T62803 NZ_CP015272:c288509-287949 [Mycobacterium intracellulare subsp. chimaera]
GTGAGCGATGCCCTCGACGAAGGGCTCGTCCAGCGCATCGACGCATGCGGCACCACTCCGTGGTCAGGCACGTGCTATCG
GTACACGACAGCTCATCGCGACGCACTCTCCGGCGAAGGCGCACGACGGTTCGGTGGCCGATGGAACCCACCACTGCTCT
TCCCCGCCATCTACCTTGCCGACTCCGCCAAGGCTTGCATGGTCGAAGTCGAACGCGCCGCCAACGCGGCCTCCACCACC
GCAATAAAGATGCTGGAGGCGGCCTACCGACTGCACCACATCCAGGTCGAGGAACTCAACGTTCTCGACTTGCGCACACC
CGAATCTCGAGACGCCGTCGGTCTCGACGACGACGACGTCTACGGTGACGACTGGTCCGCCTGCCAAGCAGTCGGACACA
CCGCCTGGTTCCTGCACATGCAAGGGATCCTGGTCCCCGCCGCCGGCGGCATCGGCCTCGTCATCTCCGCCTATGAACAG
CGAACGCGCCCAAGACAACTGCATCTCAGGCACAGCGAAAACCTCACCCCGGCGCTCTACACCCAATACCGCTCCAGCTA
A

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12612.51 Da
• Isoelectric Point: 4.8483

>AT62803 WP_042910421.1 NZ_CP015272:c288847-288506 [Mycobacterium intracellulare subsp. chimaera]
MAMNALASTVSSAIERLGLTYEEVGDIVEASPRSVARWTSGQVVPQRLNKQRLIELAYVADALAEVLPRDQANMWMFSPN
RLLEHRKPADLIRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT62803 NZ_CP015272:c288847-288506 [Mycobacterium intracellulare subsp. chimaera]
ATGGCAATGAATGCGCTCGCCTCGACCGTTTCGAGCGCCATCGAACGCCTGGGACTCACCTACGAAGAAGTCGGAGACAT
CGTCGAGGCCTCCCCACGCTCCGTGGCGCGGTGGACGTCGGGCCAAGTAGTTCCACAACGACTAAACAAGCAACGTCTGA
TCGAACTGGCTTACGTTGCCGATGCCCTTGCCGAAGTCCTCCCTCGCGACCAGGCGAATATGTGGATGTTCTCGCCGAAC
CGACTACTAGAGCACCGCAAGCCGGCCGACCTCATCCGAGATGGCGAATACCAGCGAGTGCTAGCCCTCATCGACGCCAT
GGCCGAGGGAGTGTTCGTGTGA

Structures

Toxin

• AlphaFold DB: A0A7I9ZD82

Antitoxin

• AlphaFold DB: A0A7I9ZDJ0