Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 11715..12342 Replicon plasmid unnamed 3
Accession NZ_CP015270
Organism Mycobacterium intracellulare subsp. chimaera strain ZUERICH-2

Toxin (Protein)


Gene name mazF Uniprot ID A0A854IFX7
Locus tag MYCOZU2_RS30250 Protein ID WP_054585721.1
Coordinates 11944..12342 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U5MRR7
Locus tag MYCOZU2_RS30245 Protein ID WP_054585712.1
Coordinates 11715..11951 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYCOZU2_RS30240 10101..11585 + 1485 WP_054585711.1 NAD(P)/FAD-dependent oxidoreductase -
MYCOZU2_RS30245 11715..11951 + 237 WP_054585712.1 antitoxin Antitoxin
MYCOZU2_RS30250 11944..12342 + 399 WP_054585721.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MYCOZU2_RS30255 12592..13170 + 579 WP_054585722.1 recombinase family protein -
MYCOZU2_RS30260 13389..14792 + 1404 WP_054585723.1 DUF2156 domain-containing protein -
MYCOZU2_RS31075 14891..15154 + 264 WP_139315510.1 hypothetical protein -
MYCOZU2_RS30270 15445..16149 + 705 WP_089152643.1 Crp/Fnr family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..38049 38049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14306.44 Da        Isoelectric Point: 6.9865

>T62802 WP_054585721.1 NZ_CP015270:11944-12342 [Mycobacterium intracellulare subsp. chimaera]
MPEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPAVSPAEGVDTDSVAVCRSI
RAVARTRLVERLGTIRPGTMREIEDVLTLILGIYTAQDRGEPTLRRPFLSDT

Download         Length: 399 bp

>T62802 NZ_CP015270:11944-12342 [Mycobacterium intracellulare subsp. chimaera]
TTGCCTGAGCCGCGACGGGGCGACCTCTGGCTGGTCAGCCTTGGCGCCGCTCGCGCCGGTGAGCCCGGCAAACATCGCCC
CGCCGTAGTCGTCTCCGTCGATGAGCTACTCACCGGCATCGACGACGAACTCGTCGTGGTCGTGCCAGTGTCAAGCTCCC
GCTCGCGCACCCCGCTCCGGCCAGCCGTCTCGCCCGCTGAAGGTGTGGATACTGATAGCGTTGCCGTCTGCCGCAGCATC
CGCGCAGTCGCCCGCACCCGCCTAGTGGAACGACTCGGCACCATCAGGCCCGGCACGATGCGCGAAATCGAAGATGTCCT
GACCCTCATTCTCGGCATCTACACGGCACAAGACCGTGGCGAGCCGACATTACGGCGCCCCTTCCTGAGTGACACATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8636.54 Da        Isoelectric Point: 4.8047

>AT62802 WP_054585712.1 NZ_CP015270:11715-11951 [Mycobacterium intracellulare subsp. chimaera]
MSTSTTTIRVPIQTRDRLAAQARERGISISAFLTELASQAERQAIFRVERDAIHAEAAAHAVREEDRDWEDTVGDGLA

Download         Length: 237 bp

>AT62802 NZ_CP015270:11715-11951 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTACATCCACGACGACGATCAGGGTTCCCATCCAGACGCGTGACCGCCTAGCCGCCCAAGCCCGTGAACGAGGAAT
CTCGATCTCCGCTTTCCTCACCGAACTGGCCTCCCAGGCCGAGCGCCAGGCGATCTTCCGCGTCGAACGCGACGCCATAC
ACGCCGAAGCGGCTGCCCACGCCGTGCGCGAGGAGGACCGCGATTGGGAGGACACCGTAGGCGATGGCCTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854IFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U5MRR7

References