Detailed information of TA system TA062801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 113301..113848
• Replicon: plasmid unnamed 1
• Accession: NZ_CP015268
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7U5MRA3
• Locus tag: MYCOZU2_RS29265
• Protein ID: WP_052955269.1
• Coordinates: 113567..113848 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1X0K5A9
• Locus tag: MYCOZU2_RS29260
• Protein ID: WP_047322047.1
• Coordinates: 113301..113570 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU2_RS29235	108446..109516	-	1071	WP_192579510.1	IS110 family transposase	-
MYCOZU2_RS29240	109996..110921	+	926	Protein_101	class I SAM-dependent methyltransferase	-
MYCOZU2_RS29245	110929..111162	+	234	WP_192579511.1	hypothetical protein	-
MYCOZU2_RS29250	111936..112523	+	588	WP_047322045.1	hypothetical protein	-
MYCOZU2_RS29255	112520..113185	+	666	WP_047322046.1	RES family NAD+ phosphorylase	-
MYCOZU2_RS29260	113301..113570	+	270	WP_047322047.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCOZU2_RS29265	113567..113848	+	282	WP_052955269.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYCOZU2_RS29270	114080..114937	+	858	WP_089152514.1	pentapeptide repeat-containing protein	-
MYCOZU2_RS31025	115129..115362	-	234	WP_047322049.1	hypothetical protein	-
MYCOZU2_RS29275	115451..115702	+	252	WP_089152515.1	hypothetical protein	-
MYCOZU2_RS29280	115973..116632	-	660	WP_136245930.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN	1..175972	175972
	-	flank	IS/Tn	-	-	108446..109498	1052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-90)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10672.31 Da
• Isoelectric Point: 10.2981

>T62801 WP_052955269.1 NZ_CP015268:113567-113848 [Mycobacterium intracellulare subsp. chimaera]
VSCEEASWSVHFSPTAIRALDRLPLKVAKAIAEFITGTLPTDPNRLSKPLSFEFAGWRVARRGDYRVAFRILEDDRVLLI
GRIEHRAHIYRQP

Nucleotide

• Length: 282 bp

>T62801 NZ_CP015268:113567-113848 [Mycobacterium intracellulare subsp. chimaera]
GTGAGCTGCGAAGAAGCGTCCTGGTCCGTTCACTTCTCGCCTACCGCGATCCGGGCACTCGACCGTCTACCGCTGAAAGT
CGCCAAAGCGATCGCCGAATTCATCACAGGCACACTGCCCACCGACCCGAACCGGCTATCCAAACCGTTGAGCTTCGAAT
TCGCTGGCTGGCGCGTCGCCCGGCGCGGCGATTACCGCGTCGCATTCCGGATTCTCGAAGACGACCGGGTCCTGTTGATC
GGTCGCATCGAGCACCGCGCACACATCTACCGCCAACCATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9692.77 Da
• Isoelectric Point: 4.6389

>AT62801 WP_047322047.1 NZ_CP015268:113301-113570 [Mycobacterium intracellulare subsp. chimaera]
MKNVPLSEAKDKLSALVDEADTTHEIIQITRHGRAAAVLMSADDLDSLNETLHALRTPGFAEDLAQADADYAAGNTVTGE
EIRRRYGLR

Nucleotide

• Length: 270 bp

>AT62801 NZ_CP015268:113301-113570 [Mycobacterium intracellulare subsp. chimaera]
ATGAAGAACGTACCGCTCTCGGAGGCCAAGGACAAGCTCTCCGCGCTCGTCGACGAGGCCGACACCACCCACGAAATCAT
TCAGATCACCCGACACGGGCGCGCCGCCGCGGTCCTGATGTCTGCCGACGATCTGGATTCACTCAACGAGACGCTGCACG
CACTGCGAACACCCGGTTTTGCCGAAGACCTCGCGCAGGCCGATGCCGACTACGCTGCCGGCAATACCGTCACTGGCGAG
GAGATCCGCCGGCGATACGGACTCAGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U5MRA3

Antitoxin

• AlphaFold DB: A0A1X0K5A9