Detailed information of TA system TA062797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2524314..2524978
• Replicon: chromosome
• Accession: NZ_CP015267
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: MYCOZU2_RS11885
• Protein ID: WP_009954498.1
• Coordinates: 2524571..2524978 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MYCOZU2_RS11880
• Protein ID: WP_009954496.1
• Coordinates: 2524314..2524574 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU2_RS11855	2520823..2521314	-	492	WP_089151656.1	DUF1707 domain-containing protein	-
MYCOZU2_RS11860	2521384..2521980	+	597	WP_089151657.1	TetR/AcrR family transcriptional regulator	-
MYCOZU2_RS11865	2521985..2523253	-	1269	WP_089151658.1	cytochrome P450	-
MYCOZU2_RS11870	2523411..2523863	+	453	WP_042911495.1	VOC family protein	-
MYCOZU2_RS11875	2523934..2524191	+	258	WP_042911496.1	hypothetical protein	-
MYCOZU2_RS11880	2524314..2524574	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCOZU2_RS11885	2524571..2524978	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCOZU2_RS11890	2525156..2525602	+	447	Protein_2317	DUF3427 domain-containing protein	-
MYCOZU2_RS11895	2525657..2526921	+	1265	WP_087139643.1	IS3 family transposase	-
MYCOZU2_RS11900	2526950..2529610	+	2661	Protein_2319	DUF3427 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T62797 WP_009954498.1 NZ_CP015267:2524571-2524978 [Mycobacterium intracellulare subsp. chimaera]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T62797 NZ_CP015267:2524571-2524978 [Mycobacterium intracellulare subsp. chimaera]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACTACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGGTCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCTGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT62797 WP_009954496.1 NZ_CP015267:2524314-2524574 [Mycobacterium intracellulare subsp. chimaera]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT62797 NZ_CP015267:2524314-2524574 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: J9WD27