Detailed information of TA system TA062791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 939606..940273
• Replicon: chromosome
• Accession: NZ_CP015267
• Organism: Mycobacterium intracellulare subsp. chimaera strain ZUERICH-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5PE24
• Locus tag: MYCOZU2_RS04655
• Protein ID: WP_007168407.1
• Coordinates: 939881..940273 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D5PE23
• Locus tag: MYCOZU2_RS04650
• Protein ID: WP_007168406.1
• Coordinates: 939606..939881 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCOZU2_RS31480	935110..935436	-	327	WP_033711234.1	hypothetical protein	-
MYCOZU2_RS04625	935426..935659	-	234	Protein_900	transposase	-
MYCOZU2_RS04630	935677..936888	-	1212	Protein_901	ISL3 family transposase	-
MYCOZU2_RS04635	937027..937659	-	633	Protein_902	DDE-type integrase/transposase/recombinase	-
MYCOZU2_RS30555	937611..937751	-	141	WP_145958858.1	SDR family oxidoreductase	-
MYCOZU2_RS04640	937795..938498	-	704	Protein_904	histidine phosphatase family protein	-
MYCOZU2_RS04645	938581..939453	+	873	WP_007168405.1	MaoC family dehydratase N-terminal domain-containing protein	-
MYCOZU2_RS04650	939606..939881	+	276	WP_007168406.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCOZU2_RS04655	939881..940273	+	393	WP_007168407.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCOZU2_RS04660	940421..941580	-	1160	Protein_908	trehalose-phosphatase	-
MYCOZU2_RS04665	941622..942960	-	1339	Protein_909	DUF1298 domain-containing protein	-
MYCOZU2_RS30560	943221..943343	-	123	Protein_910	IS1595 family transposase	-
MYCOZU2_RS04675	943679..945013	+	1335	Protein_911	IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-45)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14305.73 Da
• Isoelectric Point: 10.5032

>T62791 WP_007168407.1 NZ_CP015267:939881-940273 [Mycobacterium intracellulare subsp. chimaera]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRLPRVSSALARTEVMRALLHKGESARRAGRRALASLDLLRIDNRLLDLAG
GLLPIELRTLDAIHLATAQRLGMDLGRLCTYDDRMRDAAEALGMAVIAPS

Nucleotide

• Length: 393 bp

>T62791 NZ_CP015267:939881-940273 [Mycobacterium intracellulare subsp. chimaera]
ATGGCGGCCATCTACCTGGACTCGTCCGCAATCGTCAAGCTTGCAGTTCGTGAGCCAGAGTCGGATGCGCTGCGTCGCTA
CCTTCGCACCCGGCTGCCGCGGGTGTCGAGTGCCCTTGCGCGGACGGAGGTCATGCGGGCTCTGCTCCACAAGGGTGAAT
CAGCCCGAAGAGCCGGACGCCGGGCGCTCGCGAGCCTAGACCTACTACGCATCGACAACCGATTGCTCGATCTTGCTGGT
GGGCTGCTGCCAATCGAGCTACGCACCCTCGATGCAATTCACCTGGCGACTGCCCAGCGGCTCGGCATGGATCTTGGCCG
GTTGTGCACTTACGACGACCGCATGCGCGACGCCGCCGAGGCGCTCGGCATGGCAGTGATTGCCCCGTCTTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10150.63 Da
• Isoelectric Point: 5.1881

>AT62791 WP_007168406.1 NZ_CP015267:939606-939881 [Mycobacterium intracellulare subsp. chimaera]
MSTVGVRELRQRASELLRRVEAGETIEITDRGRPIALLSPMPEGGPYQQMLASGGIERATLDFDDLPEPVRLKTGIELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT62791 NZ_CP015267:939606-939881 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTACGGTGGGCGTTCGTGAGCTGCGGCAGCGGGCCAGCGAACTCCTCCGTCGGGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGTGGTCGGCCTATCGCCCTTCTCTCGCCGATGCCGGAGGGCGGTCCGTACCAGCAGATGCTGGCAAGCG
GCGGGATCGAACGTGCGACGCTTGACTTTGATGACCTTCCCGAGCCGGTCCGACTCAAAACGGGTATCGAGCTGCCGTCC
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A1X1XV84

Antitoxin

• AlphaFold DB: A0A1X1XUZ3