62788

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 234844..235486 Replicon chromosome
Accession NZ_CP015257
Organism Bacillus wiedmannii strain Bt18679

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BTI679_RS01300 Protein ID WP_000635963.1
Coordinates 235136..235486 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTI679_RS01295 Protein ID WP_000004570.1
Coordinates 234844..235131 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTI679_RS01270 (BTI679_02560) 230161..231123 + 963 WP_088048235.1 UV DNA damage repair endonuclease UvsE -
BTI679_RS01275 (BTI679_02570) 231116..231688 - 573 WP_064475233.1 rhomboid family intramembrane serine protease -
BTI679_RS01280 (BTI679_02580) 231781..232140 + 360 WP_000635044.1 holo-ACP synthase -
BTI679_RS01285 (BTI679_02590) 232297..233247 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
BTI679_RS01290 (BTI679_02600) 233366..234535 + 1170 WP_000390587.1 alanine racemase -
BTI679_RS01295 (BTI679_02610) 234844..235131 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTI679_RS01300 (BTI679_02620) 235136..235486 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTI679_RS01305 (BTI679_02630) 235555..237723 + 2169 WP_142315333.1 Tex family protein -
BTI679_RS01310 237781..237897 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BTI679_RS01315 (BTI679_02640) 238087..238551 + 465 WP_141669113.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T62788 WP_000635963.1 NZ_CP015257:235136-235486 [Bacillus wiedmannii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T62788 NZ_CP015257:235136-235486 [Bacillus wiedmannii]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT62788 WP_000004570.1 NZ_CP015257:234844-235131 [Bacillus wiedmannii]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT62788 NZ_CP015257:234844-235131 [Bacillus wiedmannii]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCGGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References