Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238807..239449 Replicon chromosome
Accession NZ_CP015250
Organism Bacillus thuringiensis Bt18247

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BTI247_RS01350 Protein ID WP_000635965.1
Coordinates 239099..239449 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTI247_RS01345 Protein ID WP_000004570.1
Coordinates 238807..239094 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTI247_RS01320 234124..235086 + 963 WP_064531740.1 UV DNA damage repair endonuclease UvsE -
BTI247_RS01325 235079..235651 - 573 WP_064531738.1 rhomboid family intramembrane serine protease -
BTI247_RS01330 235745..236104 + 360 WP_000583418.1 holo-ACP synthase -
BTI247_RS01335 236261..237211 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
BTI247_RS01340 237329..238498 + 1170 WP_069354767.1 alanine racemase -
BTI247_RS01345 238807..239094 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTI247_RS01350 239099..239449 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTI247_RS01355 239518..241686 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
BTI247_RS31295 241744..241860 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BTI247_RS01360 242056..242514 + 459 WP_069354768.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T62787 WP_000635965.1 NZ_CP015250:239099-239449 [Bacillus thuringiensis Bt18247]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T62787 NZ_CP015250:239099-239449 [Bacillus thuringiensis Bt18247]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT62787 WP_000004570.1 NZ_CP015250:238807-239094 [Bacillus thuringiensis Bt18247]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT62787 NZ_CP015250:238807-239094 [Bacillus thuringiensis Bt18247]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References