Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4637912..4638409 Replicon chromosome
Accession NZ_CP015244
Organism Escherichia coli O91 str. RM7190

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I51_RS24015 Protein ID WP_001173343.1
Coordinates 4638236..4638409 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I51_RS24010 Protein ID WP_000398619.1
Coordinates 4637912..4638208 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I51_RS23990 4634644..4635363 - 720 WP_000611288.1 two-component system response regulator DcuR -
I51_RS23995 4635360..4636991 - 1632 WP_001216487.1 sensor histidine kinase -
I51_RS24000 4637172..4637402 + 231 WP_000371697.1 (4Fe-4S)-binding protein -
I51_RS24005 4637414..4637686 + 273 WP_000405649.1 N-acetyltransferase -
I51_RS24010 4637912..4638208 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I51_RS24015 4638236..4638409 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I51_RS24020 4638528..4640045 - 1518 WP_001295088.1 lysine--tRNA ligase -
I51_RS24025 4640282..4641739 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T62781 WP_001173343.1 NZ_CP015244:4638236-4638409 [Escherichia coli O91 str. RM7190]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T62781 NZ_CP015244:4638236-4638409 [Escherichia coli O91 str. RM7190]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT62781 WP_000398619.1 NZ_CP015244:4637912-4638208 [Escherichia coli O91 str. RM7190]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT62781 NZ_CP015244:4637912-4638208 [Escherichia coli O91 str. RM7190]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References