Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4201439..4202093 Replicon chromosome
Accession NZ_CP015244
Organism Escherichia coli O91 str. RM7190

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I51_RS21825 Protein ID WP_000244777.1
Coordinates 4201686..4202093 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I51_RS21820 Protein ID WP_000354046.1
Coordinates 4201439..4201705 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I51_RS21795 4196608..4197351 + 744 WP_000951964.1 SDR family oxidoreductase -
I51_RS21800 4197408..4198841 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
I51_RS21805 4198886..4199197 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I51_RS21810 4199361..4200020 + 660 WP_000250274.1 hemolysin III family protein -
I51_RS21815 4200216..4201196 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I51_RS21820 4201439..4201705 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I51_RS21825 4201686..4202093 + 408 WP_000244777.1 toxin CptA Toxin
I51_RS21830 4202133..4202654 - 522 WP_001055874.1 flavodoxin FldB -
I51_RS21835 4202766..4203662 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I51_RS21840 4203687..4204397 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I51_RS21845 4204403..4206136 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T62779 WP_000244777.1 NZ_CP015244:4201686-4202093 [Escherichia coli O91 str. RM7190]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T62779 NZ_CP015244:4201686-4202093 [Escherichia coli O91 str. RM7190]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT62779 WP_000354046.1 NZ_CP015244:4201439-4201705 [Escherichia coli O91 str. RM7190]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT62779 NZ_CP015244:4201439-4201705 [Escherichia coli O91 str. RM7190]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References