Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3518203..3518834 Replicon chromosome
Accession NZ_CP015244
Organism Escherichia coli O91 str. RM7190

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag I51_RS18145 Protein ID WP_001259388.1
Coordinates 3518203..3518478 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag I51_RS18150 Protein ID WP_000593555.1
Coordinates 3518475..3518834 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I51_RS18130 3513207..3514274 + 1068 WP_000361483.1 HlyD family secretion protein -
I51_RS18135 3514271..3517006 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
I51_RS18140 3517006..3518130 + 1125 WP_001314210.1 ABC transporter permease -
I51_RS18145 3518203..3518478 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
I51_RS18150 3518475..3518834 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I51_RS18155 3518954..3519355 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
I51_RS18160 3519361..3520167 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
I51_RS18165 3520164..3520928 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
I51_RS18170 3520928..3521761 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
I51_RS18175 3521758..3522702 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T62776 WP_001259388.1 NZ_CP015244:3518203-3518478 [Escherichia coli O91 str. RM7190]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T62776 NZ_CP015244:3518203-3518478 [Escherichia coli O91 str. RM7190]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT62776 WP_000593555.1 NZ_CP015244:3518475-3518834 [Escherichia coli O91 str. RM7190]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT62776 NZ_CP015244:3518475-3518834 [Escherichia coli O91 str. RM7190]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References