Detailed information of TA system TA062774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2745072..2745697
• Replicon: chromosome
• Accession: NZ_CP015244
• Organism: Escherichia coli O91 str. RM7190

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I51_RS14315
• Protein ID: WP_000911330.1
• Coordinates: 2745072..2745470 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I51_RS14320
• Protein ID: WP_000450524.1
• Coordinates: 2745470..2745697 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I51_RS14295	2740979..2741179	+	201	WP_000383836.1	YpfN family protein	-
I51_RS14300	2741260..2741958	-	699	WP_000679812.1	esterase	-
I51_RS14305	2742032..2744047	-	2016	WP_000829342.1	tRNA cytosine(34) acetyltransferase TmcA	-
I51_RS14310	2744062..2744925	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
I51_RS14315	2745072..2745470	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I51_RS14320	2745470..2745697	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I51_RS14325	2745851..2746564	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I51_RS14330	2746777..2747811	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I51_RS14335	2747828..2748706	-	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I51_RS14340	2748852..2749424	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I51_RS14345	2749424..2749894	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T62774 WP_000911330.1 NZ_CP015244:c2745470-2745072 [Escherichia coli O91 str. RM7190]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T62774 NZ_CP015244:c2745470-2745072 [Escherichia coli O91 str. RM7190]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT62774 WP_000450524.1 NZ_CP015244:c2745697-2745470 [Escherichia coli O91 str. RM7190]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT62774 NZ_CP015244:c2745697-2745470 [Escherichia coli O91 str. RM7190]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33