Detailed information of TA system TA062764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1230234..1230713
• Replicon: chromosome
• Accession: NZ_CP015244
• Organism: Escherichia coli O91 str. RM7190

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0P7S0Y8
• Locus tag: I51_RS06230
• Protein ID: WP_032211256.1
• Coordinates: 1230234..1230521 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0P7PDW2
• Locus tag: I51_RS06235
• Protein ID: WP_001367155.1
• Coordinates: 1230522..1230713 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I51_RS06190	1225447..1225716	-	270	WP_000003742.1	excisionase	-
I51_RS06195	1225778..1228180	-	2403	WP_099205842.1	exonuclease	-
I51_RS06200	1228273..1228461	-	189	WP_000092783.1	DUF1482 family protein	-
I51_RS06205	1228458..1228646	-	189	WP_000450221.1	cell division inhibitor	-
I51_RS06220	1229175..1229690	+	516	WP_000559919.1	hypothetical protein	-
I51_RS06225	1229804..1229956	-	153	WP_000380318.1	DUF1391 family protein	-
I51_RS06230	1230234..1230521	-	288	WP_032211256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I51_RS06235	1230522..1230713	-	192	WP_001367155.1	hypothetical protein	Antitoxin
I51_RS06240	1230682..1231143	-	462	WP_033815696.1	helix-turn-helix domain-containing protein	-
I51_RS06245	1231163..1231534	-	372	WP_000448216.1	hypothetical protein	-
I51_RS06250	1231637..1231918	+	282	WP_021577226.1	helix-turn-helix domain-containing protein	-
I51_RS06255	1231922..1232347	+	426	WP_000693827.1	Rha family transcriptional regulator	-
I51_RS26255	1232416..1233447	+	1032	WP_052909572.1	phage replisome organizer	-
I51_RS26260	1233359..1233901	+	543	WP_072130322.1	replication protein	-
I51_RS06270	1233935..1234651	+	717	WP_047082289.1	DUF1627 domain-containing protein	-
I51_RS06275	1234684..1234965	+	282	WP_072094476.1	DNA-binding protein	-
I51_RS06280	1234962..1235267	+	306	WP_053294093.1	DUF4406 domain-containing protein	-
I51_RS06285	1235415..1235597	+	183	WP_022581296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	stx2A / stx2B	1221837..1250814	28977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11013.72 Da
• Isoelectric Point: 6.2118

>T62764 WP_032211256.1 NZ_CP015244:c1230521-1230234 [Escherichia coli O91 str. RM7190]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T62764 NZ_CP015244:c1230521-1230234 [Escherichia coli O91 str. RM7190]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACTTGGATGATATAACCAGCTATATTGCACGCTTTGACATAGA
TGCGGCAGAACGTTTATGGAAACGCTTAAGGACTTGCGTTCAGCCATTATCTGAGCATCCGTATTTATACCCGCCAAGTG
ACAGAGTGCCAGGTTTGCGTGAAATTGTAGCTCACCCCAATTACATTATTCTGTACCGAGTGACAATTTCAGGCATTGAA
ATTGTTAACGTAGTTCACGCCCGCCGACAGTTCCCTGTAACACCTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7365.28 Da
• Isoelectric Point: 5.1069

>AT62764 WP_001367155.1 NZ_CP015244:c1230713-1230522 [Escherichia coli O91 str. RM7190]
MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFAKMRKERSEQ

Nucleotide

• Length: 192 bp

>AT62764 NZ_CP015244:c1230713-1230522 [Escherichia coli O91 str. RM7190]
ATGAACACAGCCCTTTCACCGATGGTTTCTGAATTTGAAACTGTCGAGCAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGTAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTGAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0P7S0Y8

Antitoxin

• AlphaFold DB: A0A0P7PDW2