Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4790619..4791257 Replicon chromosome
Accession NZ_CP015241
Organism Escherichia coli strain 2013C-4465

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A5955_RS23750 Protein ID WP_000813794.1
Coordinates 4790619..4790795 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A5955_RS23755 Protein ID WP_001270286.1
Coordinates 4790841..4791257 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5955_RS23730 4786241..4787452 - 1212 WP_071798149.1 BenE family transporter YdcO -
A5955_RS23735 4787505..4788041 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
A5955_RS23740 4788114..4790075 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A5955_RS23745 4790167..4790397 - 231 WP_000494244.1 YncJ family protein -
A5955_RS23750 4790619..4790795 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A5955_RS23755 4790841..4791257 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A5955_RS23760 4791336..4792742 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
A5955_RS23765 4792987..4794132 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
A5955_RS23770 4794150..4795163 + 1014 WP_000220401.1 ABC transporter ATP-binding protein -
A5955_RS23775 4795164..4796105 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T62748 WP_000813794.1 NZ_CP015241:4790619-4790795 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T62748 NZ_CP015241:4790619-4790795 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT62748 WP_001270286.1 NZ_CP015241:4790841-4791257 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT62748 NZ_CP015241:4790841-4791257 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References