Detailed information of TA system TA062743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3013669..3014219
• Replicon: chromosome
• Accession: NZ_CP015241
• Organism: Escherichia coli strain 2013C-4465

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: A5955_RS14855
• Protein ID: WP_001160975.1
• Coordinates: 3013905..3014219 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: A5955_RS14850
• Protein ID: WP_000125566.1
• Coordinates: 3013669..3013902 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5955_RS14825	3008743..3009030	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
A5955_RS14830	3009088..3010419	+	1332	WP_001183177.1	MFS transporter	-
A5955_RS14835	3010527..3011057	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
A5955_RS14840	3011050..3012912	+	1863	WP_000377186.1	glutathione-regulated potassium-efflux system protein KefC	-
A5955_RS14845	3013104..3013583	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
A5955_RS14850	3013669..3013902	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A5955_RS14855	3013905..3014219	+	315	WP_001160975.1	CcdB family protein	Toxin
A5955_RS14860	3014216..3015064	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
A5955_RS14865	3015071..3015448	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
A5955_RS14870	3015451..3016272	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
A5955_RS14875	3016269..3017258	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
A5955_RS14880	3017258..3018544	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T62743 WP_001160975.1 NZ_CP015241:3013905-3014219 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T62743 NZ_CP015241:3013905-3014219 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT62743 WP_000125566.1 NZ_CP015241:3013669-3013902 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT62743 NZ_CP015241:3013669-3013902 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9