Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1790918..1791549 Replicon chromosome
Accession NZ_CP015241
Organism Escherichia coli strain 2013C-4465

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag A5955_RS09010 Protein ID WP_001259385.1
Coordinates 1791274..1791549 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A5955_RS09005 Protein ID WP_000593555.1
Coordinates 1790918..1791277 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5955_RS08975 1786030..1786503 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
A5955_RS08980 1786500..1786781 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
A5955_RS08985 1786858..1788216 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
A5955_RS08990 1788209..1789717 + 1509 WP_013009296.1 carbohydrate kinase -
A5955_RS08995 1789707..1789976 + 270 WP_000933060.1 HPr family phosphocarrier protein -
A5955_RS09000 1790008..1790868 + 861 WP_001131788.1 class II aldolase -
A5955_RS09005 1790918..1791277 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A5955_RS09010 1791274..1791549 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
A5955_RS09015 1791622..1792746 - 1125 WP_001301659.1 ABC transporter permease -
A5955_RS09020 1792746..1795481 - 2736 WP_000149105.1 ribosome-associated ATPase/putative transporter RbbA -
A5955_RS09025 1795478..1796545 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T62738 WP_001259385.1 NZ_CP015241:c1791549-1791274 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T62738 NZ_CP015241:c1791549-1791274 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT62738 WP_000593555.1 NZ_CP015241:c1791277-1790918 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT62738 NZ_CP015241:c1791277-1790918 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References