Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4304762..4305400 Replicon chromosome
Accession NZ_CP015240
Organism Escherichia coli strain 2011C-3911

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A5956_RS21830 Protein ID WP_000813794.1
Coordinates 4304762..4304938 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A5956_RS21835 Protein ID WP_001270285.1
Coordinates 4304984..4305400 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5956_RS21810 4300381..4301595 - 1215 WP_071601296.1 BenE family transporter YdcO -
A5956_RS21815 4301648..4302184 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
A5956_RS21820 4302257..4304218 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A5956_RS21825 4304310..4304540 - 231 WP_000494244.1 YncJ family protein -
A5956_RS21830 4304762..4304938 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A5956_RS21835 4304984..4305400 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A5956_RS21840 4305479..4306885 + 1407 WP_000760594.1 PLP-dependent aminotransferase family protein -
A5956_RS21845 4307130..4308275 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
A5956_RS21850 4308293..4309306 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
A5956_RS21855 4309307..4310248 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4299193..4300341 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T62721 WP_000813794.1 NZ_CP015240:4304762-4304938 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T62721 NZ_CP015240:4304762-4304938 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT62721 WP_001270285.1 NZ_CP015240:4304984-4305400 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT62721 NZ_CP015240:4304984-4305400 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References