Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1039276..1039930 Replicon chromosome
Accession NZ_CP015240
Organism Escherichia coli strain 2011C-3911

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A5956_RS05730 Protein ID WP_000244781.1
Coordinates 1039276..1039683 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A5956_RS05735 Protein ID WP_000354046.1
Coordinates 1039664..1039930 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5956_RS05710 1035233..1036966 - 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -
A5956_RS05715 1036972..1037682 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A5956_RS05720 1037707..1038603 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A5956_RS05725 1038715..1039236 + 522 WP_001055874.1 flavodoxin FldB -
A5956_RS05730 1039276..1039683 - 408 WP_000244781.1 protein YgfX Toxin
A5956_RS05735 1039664..1039930 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A5956_RS05740 1040173..1041153 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
A5956_RS05745 1041349..1042008 - 660 WP_000250274.1 hemolysin III family protein -
A5956_RS05750 1042172..1042483 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A5956_RS05755 1042528..1043961 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A5956_RS05760 1044018..1044761 - 744 WP_000951951.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T62713 WP_000244781.1 NZ_CP015240:c1039683-1039276 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T62713 NZ_CP015240:c1039683-1039276 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT62713 WP_000354046.1 NZ_CP015240:c1039930-1039664 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT62713 NZ_CP015240:c1039930-1039664 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References