Detailed information of TA system TA062704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 61042..61667
• Replicon: plasmid unnamed1
• Accession: NZ_CP015239
• Organism: Escherichia coli strain 2011C-3911

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A5956_RS00255
• Protein ID: WP_000911320.1
• Coordinates: 61269..61667 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2A2C6V4
• Locus tag: A5956_RS00250
• Protein ID: WP_000450527.1
• Coordinates: 61042..61269 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5956_RS00250	61042..61269	+	228	WP_000450527.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A5956_RS00255	61269..61667	+	399	WP_000911320.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A5956_RS00260	61676..63878	-	2203	Protein_51	type IV conjugative transfer system coupling protein TraD	-
A5956_RS00265	63934..64665	-	732	WP_047643166.1	hypothetical protein	-
A5956_RS00270	64841..65629	-	789	WP_032327001.1	hypothetical protein	-
A5956_RS00275	65797..66108	+	312	WP_000522220.1	heavy metal-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..125977	125977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T62704 WP_000911320.1 NZ_CP015239:61269-61667 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDATAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T62704 NZ_CP015239:61269-61667 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTACTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTAACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8542.54 Da
• Isoelectric Point: 4.4013

>AT62704 WP_000450527.1 NZ_CP015239:61042-61269 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSDDFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT62704 NZ_CP015239:61042-61269 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGACGATTTTATGGATAACAGGGAACAGCCCGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A2C6V4