Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/zeta-ParD
Location 997784..998730 Replicon chromosome
Accession NZ_CP015235
Organism Rhodococcus fascians D188

Toxin (Protein)


Gene name pezT Uniprot ID A0A165L952
Locus tag A3L23_RS04575 Protein ID WP_032381523.1
Coordinates 998158..998730 (+) Length 191 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID A0A161GJW3
Locus tag A3L23_RS04570 Protein ID WP_032369138.1
Coordinates 997784..998161 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3L23_RS04545 993043..994041 - 999 WP_027495727.1 substrate-binding domain-containing protein -
A3L23_RS04550 994579..995571 - 993 WP_032381576.1 YihY/virulence factor BrkB family protein -
A3L23_RS04555 995723..995893 + 171 WP_032381524.1 CsbD family protein -
A3L23_RS04560 996115..996834 + 720 WP_032366809.1 GAF and ANTAR domain-containing protein -
A3L23_RS04565 996846..997649 - 804 WP_032381575.1 cytosine methyltransferase -
A3L23_RS04570 997784..998161 + 378 WP_032369138.1 hypothetical protein Antitoxin
A3L23_RS04575 998158..998730 + 573 WP_032381523.1 zeta toxin family protein Toxin
A3L23_RS04580 998746..1000188 - 1443 WP_032369140.1 PepSY domain-containing protein -
A3L23_RS04585 1000335..1000865 - 531 WP_052054639.1 hypothetical protein -
A3L23_RS04590 1001010..1001270 + 261 WP_141214541.1 hypothetical protein -
A3L23_RS04595 1001319..1002473 + 1155 WP_080729090.1 hypothetical protein -
A3L23_RS04600 1002443..1003081 - 639 WP_052054644.1 DUF1275 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(40-142)

Antitoxin

ParD-like antitoxin of type II bacterial toxin-antitoxin system

(6-61)


Sequences

Toxin        


Download         Length: 191 a.a.        Molecular weight: 20858.92 Da        Isoelectric Point: 9.3426

>T62700 WP_032381523.1 NZ_CP015235:998158-998730 [Rhodococcus fascians D188]
VKRLDLVIGPNGSGKTTFVEQRLLALLPLSAFVNADVIAKQNWPRSPEAHSYEAAKVAAQLRTALVDAGESFIAETVFSH
PSKLEFATAAKQAGYVIVVHAIMVPVELSIARVAVRVEEGGHHVPEVKIRERYARLWPLVAKAIELADHATVYDNSAPDR
TRIAARYVDGDVVGDTHWPPWTPAALTRRR

Download         Length: 573 bp

>T62700 NZ_CP015235:998158-998730 [Rhodococcus fascians D188]
GTGAAACGCCTCGATCTCGTCATCGGCCCCAATGGATCCGGCAAAACGACGTTCGTCGAGCAGCGCCTGCTTGCACTCCT
CCCGCTCAGCGCCTTCGTCAACGCGGATGTCATCGCCAAGCAGAACTGGCCGCGATCCCCGGAGGCACACTCCTACGAGG
CAGCGAAGGTGGCCGCTCAGCTGCGCACTGCACTCGTGGACGCCGGCGAATCGTTCATCGCCGAGACTGTGTTCTCACAT
CCCTCGAAACTCGAATTCGCTACCGCCGCAAAACAAGCAGGCTATGTGATCGTGGTCCACGCGATCATGGTTCCGGTCGA
ACTGTCGATTGCCCGCGTCGCAGTGCGGGTCGAGGAGGGTGGGCACCACGTACCCGAGGTCAAGATCCGCGAGAGGTACG
CGAGACTGTGGCCACTGGTCGCGAAGGCCATCGAACTGGCCGACCACGCGACCGTGTACGACAACTCGGCCCCGGACAGG
ACCCGTATCGCCGCGCGATACGTCGACGGCGACGTCGTCGGCGATACCCACTGGCCGCCTTGGACACCGGCGGCGCTCAC
GCGCCGCCGGTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13797.30 Da        Isoelectric Point: 5.6062

>AT62700 WP_032369138.1 NZ_CP015235:997784-998161 [Rhodococcus fascians D188]
MTNVADRVTRFSSDLVDDAAAEGERENRSARQQLEHWARVGREVSSQRAVARRRVESALLGRTPLAELTPEEGVVFNAEV
AAAIEESMATGHHLEERTAQGRVSVFLDEHGRVVKHLPDGTQTVL

Download         Length: 378 bp

>AT62700 NZ_CP015235:997784-998161 [Rhodococcus fascians D188]
ATGACGAACGTCGCCGATCGCGTCACACGTTTCTCGTCGGACCTCGTCGATGACGCCGCCGCAGAGGGCGAGCGCGAGAA
TCGCTCTGCCAGGCAACAATTGGAGCACTGGGCAAGAGTAGGCCGCGAGGTGTCGAGCCAGCGGGCGGTCGCACGCCGCC
GCGTCGAGTCCGCACTCCTGGGTCGCACGCCCCTTGCCGAACTCACACCCGAGGAGGGTGTCGTGTTCAATGCCGAGGTT
GCCGCAGCCATCGAGGAGTCGATGGCTACCGGACATCATCTCGAAGAACGCACCGCACAGGGACGCGTGTCGGTATTCCT
CGACGAGCACGGACGGGTCGTCAAGCATCTTCCGGACGGTACGCAGACAGTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A165L952

Antitoxin

Source ID Structure
AlphaFold DB A0A161GJW3

References