Detailed information of TA system TA062698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4899607..4900223
• Replicon: chromosome
• Accession: NZ_CP015229
• Organism: Escherichia coli strain 06-00048

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A176ZIX8
• Locus tag: GJ11_RS26060
• Protein ID: WP_001129490.1
• Coordinates: 4899849..4900223 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GJ11_RS26055
• Protein ID: WP_065227998.1
• Coordinates: 4899607..4899849 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS26035	4894842..4895279	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GJ11_RS26040	4895324..4896265	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GJ11_RS26045	4896681..4898495	+	1815	WP_065227996.1	hypothetical protein	-
GJ11_RS26050	4899171..4899389	+	219	WP_004019319.1	ribbon-helix-helix domain-containing protein	-
GJ11_RS26055	4899607..4899849	+	243	WP_065227998.1	ribbon-helix-helix domain-containing protein	Antitoxin
GJ11_RS26060	4899849..4900223	+	375	WP_001129490.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJ11_RS26065	4900260..4901189	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GJ11_RS26070	4901186..4901821	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GJ11_RS26075	4901818..4902720	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-48)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13960.19 Da
• Isoelectric Point: 7.0126

>T62698 WP_001129490.1 NZ_CP015229:4899849-4900223 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Nucleotide

• Length: 375 bp

>T62698 NZ_CP015229:4899849-4900223 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9304.57 Da
• Isoelectric Point: 4.3894

>AT62698 WP_065227998.1 NZ_CP015229:4899607-4899849 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT62698 NZ_CP015229:4899607-4899849 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGAACAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A176ZIX8