Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3691529..3692183 Replicon chromosome
Accession NZ_CP015229
Organism Escherichia coli strain 06-00048

Toxin (Protein)


Gene name cptA Uniprot ID A0A193LWV0
Locus tag GJ11_RS20285 Protein ID WP_065227581.1
Coordinates 3691529..3691936 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GJ11_RS20290 Protein ID WP_000354046.1
Coordinates 3691917..3692183 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS20265 3687486..3689219 - 1734 WP_065227579.1 single-stranded-DNA-specific exonuclease RecJ -
GJ11_RS20270 3689225..3689935 - 711 WP_065227580.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GJ11_RS20275 3689960..3690856 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GJ11_RS20280 3690968..3691489 + 522 WP_001055864.1 flavodoxin FldB -
GJ11_RS20285 3691529..3691936 - 408 WP_065227581.1 protein YgfX Toxin
GJ11_RS20290 3691917..3692183 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GJ11_RS20295 3692426..3693406 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
GJ11_RS20300 3693483..3694142 - 660 WP_065227582.1 hemolysin III family protein -
GJ11_RS20305 3694306..3694617 - 312 WP_065227583.1 N(4)-acetylcytidine aminohydrolase -
GJ11_RS20310 3694662..3696095 + 1434 WP_065227584.1 6-phospho-beta-glucosidase BglA -
GJ11_RS20315 3696157..3696900 - 744 WP_065227585.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16001.93 Da        Isoelectric Point: 11.5202

>T62695 WP_065227581.1 NZ_CP015229:c3691936-3691529 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQEAQR

Download         Length: 408 bp

>T62695 NZ_CP015229:c3691936-3691529 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGGCGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT62695 WP_000354046.1 NZ_CP015229:c3692183-3691917 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT62695 NZ_CP015229:c3692183-3691917 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCGGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193LWV0

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References