Detailed information of TA system TA062682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1771264..1771902
• Replicon: chromosome
• Accession: NZ_CP015229
• Organism: Escherichia coli strain 06-00048

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A193LRF3
• Locus tag: GJ11_RS10545
• Protein ID: WP_065226776.1
• Coordinates: 1771264..1771440 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GJ11_RS10550
• Protein ID: WP_077884271.1
• Coordinates: 1771486..1771902 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS10525	1766886..1768097	-	1212	WP_071906111.1	BenE family transporter YdcO	-
GJ11_RS10530	1768150..1768686	+	537	WP_065226774.1	DNA-binding transcriptional regulator SutR	-
GJ11_RS10535	1768759..1770720	+	1962	WP_065226775.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GJ11_RS10540	1770812..1771042	-	231	WP_000494241.1	YncJ family protein	-
GJ11_RS10545	1771264..1771440	+	177	WP_065226776.1	type II toxin-antitoxin system HicA family toxin	Toxin
GJ11_RS10550	1771486..1771902	+	417	WP_077884271.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GJ11_RS10555	1771981..1773387	+	1407	WP_065226778.1	PLP-dependent aminotransferase family protein	-
GJ11_RS10560	1773632..1774777	+	1146	WP_065226779.1	ABC transporter substrate-binding protein	-
GJ11_RS10565	1774795..1775808	+	1014	WP_065226780.1	ABC transporter ATP-binding protein	-
GJ11_RS10570	1775809..1776750	+	942	WP_065226781.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6752.77 Da
• Isoelectric Point: 11.5111

>T62682 WP_065226776.1 NZ_CP015229:1771264-1771440 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKESLRQAILKLLSLS

Nucleotide

• Length: 177 bp

>T62682 NZ_CP015229:1771264-1771440 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTAAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAATCATTGCGTCAAGCCATTCTGAAAC
TACTCAGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15231.66 Da
• Isoelectric Point: 4.7386

>AT62682 WP_077884271.1 NZ_CP015229:1771486-1771902 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQLELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT62682 NZ_CP015229:1771486-1771902 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCTAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A193LRF3