Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 569933..570551 Replicon chromosome
Accession NZ_CP015229
Organism Escherichia coli strain 06-00048

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GJ11_RS04640 Protein ID WP_001291435.1
Coordinates 569933..570151 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A193LN30
Locus tag GJ11_RS04645 Protein ID WP_000344802.1
Coordinates 570177..570551 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS04605 565222..565794 + 573 WP_065226294.1 YbaY family lipoprotein -
GJ11_RS04610 565825..566136 - 312 WP_065226295.1 MGMT family protein -
GJ11_RS04620 566515..566868 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GJ11_RS04625 566910..568460 - 1551 WP_065228190.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GJ11_RS04630 568624..569094 - 471 WP_000136192.1 YlaC family protein -
GJ11_RS04635 569210..569761 - 552 WP_065226296.1 maltose O-acetyltransferase -
GJ11_RS04640 569933..570151 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GJ11_RS04645 570177..570551 - 375 WP_000344802.1 Hha toxicity modulator TomB Antitoxin
GJ11_RS04650 571097..574246 - 3150 WP_001132468.1 multidrug efflux RND transporter permease subunit -
GJ11_RS04655 574269..575462 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62672 WP_001291435.1 NZ_CP015229:c570151-569933 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62672 NZ_CP015229:c570151-569933 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14587.42 Da        Isoelectric Point: 4.7395

>AT62672 WP_000344802.1 NZ_CP015229:c570551-570177 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNTTKENPASLSC

Download         Length: 375 bp

>AT62672 NZ_CP015229:c570551-570177 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATACGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A193LN30

References