Detailed information of TA system TA062671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 341444..343133
• Replicon: chromosome
• Accession: NZ_CP015229
• Organism: Escherichia coli strain 06-00048

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A193LMX0
• Locus tag: GJ11_RS03575
• Protein ID: WP_065226193.1
• Coordinates: 342213..343133 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: GJ11_RS03570
• Protein ID: WP_065226192.1
• Coordinates: 341444..342226 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS03545	336755..336958	-	204	Protein_311	integrase	-
GJ11_RS03550	337005..337107	-	103	Protein_312	helix-turn-helix domain-containing protein	-
GJ11_RS03555	337104..337574	-	471	WP_065226190.1	hypothetical protein	-
GJ11_RS03560	337625..337738	-	114	WP_157921667.1	ash family protein	-
GJ11_RS03565	337918..341127	-	3210	WP_065226191.1	RNA-directed DNA polymerase	-
GJ11_RS03570	341444..342226	+	783	WP_065226192.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GJ11_RS03575	342213..343133	+	921	WP_065226193.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GJ11_RS03580	343539..344438	-	900	WP_065226194.1	LysR family transcriptional regulator	-
GJ11_RS03585	344537..345259	+	723	WP_065226195.1	SDR family oxidoreductase	-
GJ11_RS03590	345423..345701	+	279	WP_065226196.1	hypothetical protein	-
GJ11_RS03595	346528..347412	-	885	WP_001119037.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	337104..356019	18915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34729.08 Da
• Isoelectric Point: 6.6441

>T62671 WP_065226193.1 NZ_CP015229:342213-343133 [Escherichia coli]
MDLRDSYTRQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKTFADRDTDLTAINDALKRITASLNSK
PGITAIRQENKTDEKRIIVNTADAQIKIEVSPVWRGLLLPPAEMSVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T62671 NZ_CP015229:342213-343133 [Escherichia coli]
ATGGATCTGCGTGATAGCTATACCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACTTTTGCGGATCGCGATACCGATTTGACGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATTACCGCGATTCGGCAGGAAAATAAAACGGATGAAAAGCGCATCATTGTGAATACGGCTGATGCCCAGATTAA
GATCGAGGTTAGTCCGGTCTGGCGTGGGCTGTTGTTGCCTCCGGCAGAAATGTCGGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACTATGAGTGTCGTTTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCCC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29162.37 Da
• Isoelectric Point: 7.7553

>AT62671 WP_065226192.1 NZ_CP015229:341444-342226 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQIWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPREFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT62671 NZ_CP015229:341444-342226 [Escherichia coli]
TTGCCAAATTATCTAAACTGGCTGATGCAGAATACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTCTCTTACCTCTATGTCCAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGACTGGGTGGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCAGTACGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAAATCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCA
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATATCGTTCGAACATGCCGATGCTCTGTTTCAGGGGATGAATCTTCTCAGCCCGCGTAAATT
GCAGGCGTTACTCTGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGAATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A193LMX0